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Stochastic and reversible assembly of a multiprotein DNA repair complex ensures accurate target site recognition and efficient repair

To understand how multiprotein complexes assemble and function on chromatin, we combined quantitative analysis of the mammalian nucleotide excision DNA repair (NER) machinery in living cells with computational modeling. We found that individual NER components exchange within tens of seconds between...

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Main Authors: Luijsterburg, Martijn (Author) , Terstiege, Gesa (Author) , Gourdin, Audrey M. (Author) , Politi, Antonio Z. (Author) , Moné, Martijn J. (Author) , Warmerdam, Daniël O. (Author) , Goedhart, Joachim (Author) , Vermeulen, Wim (Author) , van Driel, Roel (Author) , Höfer, Thomas (Author)
Format: Article (Journal)
Language:English
Published: May 03 2010
In: The journal of cell biology
Year: 2010, Volume: 189, Issue: 3, Pages: 445-463
ISSN:1540-8140
DOI:10.1083/jcb.200909175
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1083/jcb.200909175
Verlag, lizenzpflichtig, Volltext: https://rupress.org/jcb/article/189/3/445/35981/Stochastic-and-reversible-assembly-of-a
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Author Notes:Martijn S. Luijsterburg, Gesa von Bornstaedt, Audrey M. Gourdin, Antonio Z. Politi, Martijn J. Moné, Daniël O. Warmerdam, Joachim Goedhart, Wim Vermeulen, Roel van Driel, and Thomas Höfer
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Summary:To understand how multiprotein complexes assemble and function on chromatin, we combined quantitative analysis of the mammalian nucleotide excision DNA repair (NER) machinery in living cells with computational modeling. We found that individual NER components exchange within tens of seconds between the bound state in repair complexes and the diffusive state in the nucleoplasm, whereas their net accumulation at repair sites evolves over several hours. Based on these in vivo data, we developed a predictive kinetic model for the assembly and function of repair complexes. DNA repair is orchestrated by the interplay of reversible protein-binding events and progressive enzymatic modifications of the chromatin substrate. We demonstrate that faithful recognition of DNA lesions is time consuming, whereas subsequently, repair complexes form rapidly through random and reversible assembly of NER proteins. Our kinetic analysis of the NER system reveals a fundamental conflict between specificity and efficiency of chromatin-associated protein machineries and shows how a trade off is negotiated through reversibility of protein binding.
Item Description:Gesehen am 19.04.2023
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.200909175

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