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Distance between homologous chromosomes results from chromosome positioning constraints

The organization of chromosomes is important for various biological processes and is involved in the formation of rearrangements often observed in cancer. In mammals, chromosomes are organized in territories that are radially positioned in the nucleus. However, it remains unclear whether chromosomes...

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Bibliographische Detailangaben
Hauptverfasser: Heride, Claire (VerfasserIn) , Ricoul, Michelle (VerfasserIn) , Kiêu, Kien (VerfasserIn) , Hase, Johann von (VerfasserIn) , Guillemot, Vincent (VerfasserIn) , Cremer, Christoph (VerfasserIn) , Dubrana, Karine (VerfasserIn) , Sabatier, Laure (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 01 December 2010
In: Journal of cell science
Year: 2010, Jahrgang: 123, Heft: 23, Pages: 4063-4075
ISSN:1477-9137
DOI:10.1242/jcs.066498
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1242/jcs.066498
Volltext
Verfasserangaben:Claire Heride, Michelle Ricoul, Kien Kiêu, Johann von Hase, Vincent Guillemot, Christoph Cremer, Karine Dubrana and Laure Sabatier
Beschreibung
Zusammenfassung:The organization of chromosomes is important for various biological processes and is involved in the formation of rearrangements often observed in cancer. In mammals, chromosomes are organized in territories that are radially positioned in the nucleus. However, it remains unclear whether chromosomes are organized relative to each other. Here, we examine the nuclear arrangement of 10 chromosomes in human epithelial cancer cells by three-dimensional FISH analysis. We show that their radial position correlates with the ratio of their gene density to chromosome size. We also observe that inter-homologue distances are generally larger than inter-heterologue distances. Using numerical simulations taking radial position constraints into account, we demonstrate that, for some chromosomes, radial position is enough to justify the inter-homologue distance, whereas for others additional constraints are involved. Among these constraints, we propose that nucleolar organizer regions participate in the internal positioning of the acrocentric chromosome HSA21, possibly through interactions with nucleoli. Maintaining distance between homologous chromosomes in human cells could participate in regulating genome stability and gene expression, both mechanisms that are key players in tumorigenesis.
Beschreibung:Gesehen am 26.04.2023
Beschreibung:Online Resource
ISSN:1477-9137
DOI:10.1242/jcs.066498

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