A simple fluorescence based assay for quantification of human immunodeficiency virus particle release
The assembly and release of human immunodeficiency virus (HIV) particles from infected cells represent attractive, but not yet exploited targets for antiretroviral therapy. The availability of simple methods to measure the efficiency of these replication steps in tissue culture would facilitate the...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
20 April 2010
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| In: |
BMC biotechnology
Year: 2010, Volume: 10, Pages: 1-12 |
| ISSN: | 1472-6750 |
| DOI: | 10.1186/1472-6750-10-32 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1186/1472-6750-10-32
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| Author Notes: | Johannes Hermle, Maria Anders, Anke-Mareil Heuser and Barbara Müller |
| Summary: | The assembly and release of human immunodeficiency virus (HIV) particles from infected cells represent attractive, but not yet exploited targets for antiretroviral therapy. The availability of simple methods to measure the efficiency of these replication steps in tissue culture would facilitate the identification of host factors essential for these processes as well as the screening for lead compounds acting as specific inhibitors of particle formation. We describe here the development of a rapid cell based assay for quantification of human immunodeficiency virus type 1 (HIV-1) particle assembly and/or release. |
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| Item Description: | Gesehen am 26.04.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1472-6750 |
| DOI: | 10.1186/1472-6750-10-32 |