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Retinal overexpression of angiopoietin-2 mimics diabetic retinopathy and enhances vascular damages in hyperglycemia

Our previous data suggested that angiopoietin-2 (Ang-2) is linked to pericyte loss, thereby playing an important role in diabetic retinopathy. In this study, we investigated the effect of retinal overexpression of human Ang-2 (mOpsinhAng2 mouse) on vascular morphology in non-diabetic and streptozoto...

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Main Authors: Pfister, Frederick (Author) , Wang, Yumei (Author) , Schreiter, Kay (Author) , vom Hagen, Franziska (Author) , Altvater, Karin (Author) , Hoffmann, Sigrid (Author) , Deutsch, Urban (Author) , Hammes, Hans-Peter (Author) , Feng, Yuxi (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: Acta diabetologica
Year: 2010, Volume: 47, Pages: 59-64$i6
ISSN:1432-5233
DOI:10.1007/s00592-009-0099-2
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s00592-009-0099-2
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Author Notes:Frederick Pfister, Yumei Wang, Kay Schreiter, Franziska vom Hagen, Karin Altvater, Sigrid Hoffmann, Urban Deutsch, Hans-Peter Hammes, Yuxi Feng
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Summary:Our previous data suggested that angiopoietin-2 (Ang-2) is linked to pericyte loss, thereby playing an important role in diabetic retinopathy. In this study, we investigated the effect of retinal overexpression of human Ang-2 (mOpsinhAng2 mouse) on vascular morphology in non-diabetic and streptozotozin-induced diabetic animals. Pericyte (PC) coverage and acellular capillary (AC) formation were quantitated in retinal digest preparations after 3 and 6 months of diabetes duration. The degree of retinopathy in non-diabetic mOpsinhAng2 mice at 3 months (−21% PC, +49% AC) was comparable to age-matched diabetic wild type mice. Diabetic mOpsinhAng2 mice exhibited significantly worse vascular pathology than wild type counterparts at 6 months. Quantitative PCR revealed that human Ang-2 mRNA was highly overexpressed in retinas of transgenic mice. Our data demonstrate that overexpression of Ang-2 in the retina enhances vascular pathology, indicating that Ang-2 plays an essential role in diabetic vasoregression via destabilization of pericytes.
Item Description:Online veröffentlicht: 24. Februar 2009
Gesehen am 02.05.2023
Physical Description:Online Resource
ISSN:1432-5233
DOI:10.1007/s00592-009-0099-2

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