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A simple approach for detecting HLA-A*02 alleles in archival formalin-fixed paraffin-embedded tissue samples and an application example for studying cancer immunoediting

The HLA system represents a central component of the antigen presentation machinery. As every patient possesses a defined set of HLA molecules, only certain antigens can be presented on the cell surface. Thus, studying HLA type-dependent antigen presentation can improve the understanding of variatio...

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Main Authors: Witt, Johannes (Author) , Haupt, Saskia (Author) , Ahadova, Aysel (Author) , Bohaumilitzky, Lena (Author) , Fuchs, Vera (Author) , Ballhausen, Alexej (Author) , Przybilla, Moritz Jakob (Author) , Jendrusch, Michael (Author) , Seppälä, Toni T. (Author) , Fürst, Daniel (Author) , Walle, Thomas (Author) , Busch, Elena (Author) , Haag, Georg Martin (Author) , Hüneburg, Robert (Author) , Nattermann, Jacob (Author) , Knebel Doeberitz, Magnus von (Author) , Heuveline, Vincent (Author) , Kloor, Matthias (Author)
Format: Article (Journal)
Language:English
Published: January 2023
In: HLA
Year: 2023, Volume: 101, Issue: 1, Pages: 24-33
ISSN:2059-2310
DOI:10.1111/tan.14846
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1111/tan.14846
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/tan.14846
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Author Notes:Johannes Witt, Saskia Haupt, Aysel Ahadova, Lena Bohaumilitzky, Vera Fuchs, Alexej Ballhausen, Moritz Jakob Przybilla, Michael Jendrusch, Toni T. Seppälä, Daniel Fürst, Thomas Walle, Elena Busch, Georg Martin Haag, Robert Hüneburg, Jacob Nattermann, Magnus von Knebel Doeberitz, Vincent Heuveline, Matthias Kloor
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Summary:The HLA system represents a central component of the antigen presentation machinery. As every patient possesses a defined set of HLA molecules, only certain antigens can be presented on the cell surface. Thus, studying HLA type-dependent antigen presentation can improve the understanding of variation in susceptibility to various diseases, including infectious diseases and cancer. In archival formalin-fixed paraffin-embedded (FFPE) tissue, the HLA type is difficult to analyze because of fragmentation of DNA, hindering the application of commonly used assays that rely on long DNA stretches. Addressing these difficulties, we present a refined approach for characterizing presence or absence of HLA-A*02, the most common HLA-A allele in the Caucasian population, in archival samples. ...
Item Description:Online veröffentlicht: 17. Oktober 2022
Gesehen am 22.11.2023
Physical Description:Online Resource
ISSN:2059-2310
DOI:10.1111/tan.14846

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