Cover Image

Screening for diverse PDGFRA or PDGFRB fusion genes is facilitated by generic quantitative reverse transcriptase polymerase chain reaction analysis

Background Rapid identification of diverse fusion genes with involvement of PDGFRA or PDGFRB in eosinophilia-associated myeloproliferative neoplasms is essential for adequate clinical management but is complicated by the multitude and heterogeneity of partner genes and breakpoints.Design and Methods...

Full description

Saved in:
Bibliographic Details
Main Authors: Erben, Philipp (Author) , Gosenca, Darko (Author) , Müller, Martin Christian (Author) , Reiter, Jelena Katharina (Author) , Score, Joannah (Author) , Valle, Francesco del (Author) , Walz, Christoph (Author) , Mix, Jürgen (Author) , Metzgeroth, Georgia (Author) , Ernst, Thomas (Author) , Haferlach, Claudia (Author) , Cross, Nicholas C. P. (Author) , Hochhaus, Andreas (Author) , Reiter, Andreas (Author)
Format: Article (Journal)
Language:English
Published: 2010-05-01
In: Haematologica, the hematology journal
Year: 2010, Volume: 95, Issue: 5, Pages: 738-744
ISSN:1592-8721
DOI:10.3324/haematol.2009.016345
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.3324/haematol.2009.016345
Verlag, lizenzpflichtig, Volltext: https://haematologica.org/article/view/5585
Get full text
Author Notes:Philipp Erben, Darko Gosenca, Martin C. Müller, Jelena Reinhard, Joannah Score, Francesco del Valle, Christoph Walz, Jürgen Mix, Georgia Metzgeroth, Thomas Ernst, Claudia Haferlach, Nicholas C. P. Cross, Andreas Hochhaus, and Andreas Reiter
Description
Summary:Background Rapid identification of diverse fusion genes with involvement of PDGFRA or PDGFRB in eosinophilia-associated myeloproliferative neoplasms is essential for adequate clinical management but is complicated by the multitude and heterogeneity of partner genes and breakpoints.Design and Methods We established a generic quantitative reverse transcriptase polymerase chain reaction to detect overexpression of the 3′-regions of PDGFRA or PDGFRB as a possible indicator of an underlying fusion.Results At diagnosis, all patients with known fusion genes involving PDGFRA (n=5; 51 patients) or PDGFRB (n=5; 7 patients) showed significantly increased normalized expression levels compared to 191 patients with fusion gene-negative eosinophilia or healthy individuals (PDGFRA/ABL: 0.73 versus 0.0066 versus 0.0064, P
Item Description:Gesehen am 25.05.2023
Physical Description:Online Resource
ISSN:1592-8721
DOI:10.3324/haematol.2009.016345

Similar Items