Human dendritic cells contain cell surface sialyltransferase activity
Human monocyte-derived dendritic cells (mo-DCs) express highly sialylated structures, with recognized but poorly understood function in maturation, immunogenicity and endocytosis capacity. We have previously shown that mo-DCs surface sialylation is changeable upon different stimuli, which led us to...
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| Main Authors: | , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
3 March 2010
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| In: |
Immunology letters
Year: 2010, Volume: 131, Issue: 1, Pages: 89-96 |
| ISSN: | 1879-0542 |
| DOI: | 10.1016/j.imlet.2010.02.009 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.imlet.2010.02.009 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0165247810000775 
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| Author Notes: | M. Guadalupe Cabral, A. Rita Piteira, Zélia Silva, Dário Ligeiro, Reinhard Brossmer, Paula A. Videira |
| Summary: | Human monocyte-derived dendritic cells (mo-DCs) express highly sialylated structures, with recognized but poorly understood function in maturation, immunogenicity and endocytosis capacity. We have previously shown that mo-DCs surface sialylation is changeable upon different stimuli, which led us to hypothesise the existence of cell surface (non-intracellular) sialyltransferases, rapidly restoring or altering mo-DC surface sialylation, thus modulating specific functions. Here, we demonstrate that, in the presence of exogenous CMP-Neu5Ac, mo-DCs incorporate considerable amounts of sialic acids into cell surface, predominantly when mo-DCs were previously desialylated or matured. This is a genuine sialyltransferase activity, confirmed by specific inhibition assays, which is not influenced by secreted enzymes. Functionally, the ecto-sialyltransferase activity causes a significant down-regulation of mo-DCs endocytic capacity, without affecting the maturation state. These findings suggest that ecto-sialyltransferases participate in a dynamic control of mo-DC sialylation, with functional repercussions. This activity is possibly related with specific physiological and pathological conditions, as inflammation and infection, contributing to protection and homeostasis regulation. |
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| Item Description: | Gesehen am 01.06.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0542 |
| DOI: | 10.1016/j.imlet.2010.02.009 |