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Generation of two homozygous SHOX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9

SHOX2 is a homeobox transcription factor associated with atrial fibrillation (AF) and sinus node dysfunction. Here, we generated two homozygous SHOX2 knock-out hiPSC lines from a healthy control line and a corrected AF patient line (disease-specific SHOX2 mutation corrected to WT) using CRISPR/Cas9....

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Main Authors: Rädecke, Kristin (Author) , Gore, Ambuj (Author) , Burau, Karin (Author) , Laugsch, Magdalena (Author) , Köhler, Katrin (Author) , Rappold, Gudrun (Author) , Hoffmann, Sandra (Author)
Format: Article (Journal)
Language:English
Published: 5 April 2023
In: Stem cell research
Year: 2023, Volume: 69, Pages: 1-7
ISSN:1876-7753
DOI:10.1016/j.scr.2023.103089
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.scr.2023.103089
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1873506123000752
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Author Notes:Kristin Rädecke, Ambuj Gore, Karin Burau, Magdalena Laugsch, Katrin Köhler, Gudrun A Rappold, Sandra Hoffmann
Description
Summary:SHOX2 is a homeobox transcription factor associated with atrial fibrillation (AF) and sinus node dysfunction. Here, we generated two homozygous SHOX2 knock-out hiPSC lines from a healthy control line and a corrected AF patient line (disease-specific SHOX2 mutation corrected to WT) using CRISPR/Cas9. These cell lines maintained pluripotency, an ability to differentiate into all three germlayers and a normal karyotype, presenting a valuable tool to investigate the impact of a full SHOX2 knock-out with respect to arrhythmogenic diseases on a cellular level.
Item Description:Online verfügbar 30. März 2023, Artikelversion 5. April 2023
Gesehen am 13.06.2023
Physical Description:Online Resource
ISSN:1876-7753
DOI:10.1016/j.scr.2023.103089

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