Generation of two homozygous SHOX2 knock-out human induced pluripotent stem cell lines using CRISPR/Cas9
SHOX2 is a homeobox transcription factor associated with atrial fibrillation (AF) and sinus node dysfunction. Here, we generated two homozygous SHOX2 knock-out hiPSC lines from a healthy control line and a corrected AF patient line (disease-specific SHOX2 mutation corrected to WT) using CRISPR/Cas9....
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
5 April 2023
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| In: |
Stem cell research
Year: 2023, Jahrgang: 69, Pages: 1-7 |
| ISSN: | 1876-7753 |
| DOI: | 10.1016/j.scr.2023.103089 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.scr.2023.103089 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1873506123000752 
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| Verfasserangaben: | Kristin Rädecke, Ambuj Gore, Karin Burau, Magdalena Laugsch, Katrin Köhler, Gudrun A Rappold, Sandra Hoffmann |
| Zusammenfassung: | SHOX2 is a homeobox transcription factor associated with atrial fibrillation (AF) and sinus node dysfunction. Here, we generated two homozygous SHOX2 knock-out hiPSC lines from a healthy control line and a corrected AF patient line (disease-specific SHOX2 mutation corrected to WT) using CRISPR/Cas9. These cell lines maintained pluripotency, an ability to differentiate into all three germlayers and a normal karyotype, presenting a valuable tool to investigate the impact of a full SHOX2 knock-out with respect to arrhythmogenic diseases on a cellular level. |
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| Beschreibung: | Online verfügbar 30. März 2023, Artikelversion 5. April 2023 Gesehen am 13.06.2023 |
| Beschreibung: | Online Resource |
| ISSN: | 1876-7753 |
| DOI: | 10.1016/j.scr.2023.103089 |