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Direct observation of motor protein stepping in living cells using MINFLUX

Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dime...

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Main Authors: Deguchi, Takahiro (Author) , Iwanski, Malina K. (Author) , Schentarra, Eva-Maria (Author) , Heidebrecht, Christopher (Author) , Schmidt, Lisa (Author) , Heck, Jennifer (Author) , Weihs, Tobias (Author) , Schnorrenberg, Sebastian (Author) , Hoess, Philipp (Author) , Liu, Sheng (Author) , Chevyreva, Veronika (Author) , Noh, Kyung-Min (Author) , Kapitein, Lukas C. (Author) , Ries, Jonas (Author)
Format: Article (Journal)
Language:English
Published: 9 Mar 2023
In: Science
Year: 2023, Volume: 379, Issue: 6636, Pages: 1010-1015
ISSN:1095-9203
DOI:10.1126/science.ade2676
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1126/science.ade2676
Verlag, lizenzpflichtig, Volltext: https://www.science.org/doi/10.1126/science.ade2676
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Author Notes:Takahiro Deguchi, Malina K. Iwanski, Eva-Maria Schentarra, Christopher Heidebrecht, Lisa Schmidt, Jennifer Heck, Tobias Weihs, Sebastian Schnorrenberg, Philipp Hoess, Sheng Liu, Veronika Chevyreva, Kyung-Min Noh, Lukas C. Kapitein, Jonas Ries
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Summary:Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
Item Description:Gesehen am 21.06.2023
Physical Description:Online Resource
ISSN:1095-9203
DOI:10.1126/science.ade2676

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