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Binding partner switching on microtubules and Aurora-B in the mitosis to cytokinesis transition

The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that...

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Hauptverfasser: Özlü, Nurhan (VerfasserIn) , Monigatti, Flavio (VerfasserIn) , Renard, Bernhard Y. (VerfasserIn) , Field, Christine M. (VerfasserIn) , Steen, Hanno (VerfasserIn) , Mitchison, Timothy J. (VerfasserIn) , Steen, Judith J. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 2010
In: Molecular & cellular proteomics
Year: 2010, Jahrgang: 9, Heft: 2, Pages: 336-350
ISSN:1535-9484
DOI:10.1074/mcp.M900308-MCP200
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1074/mcp.M900308-MCP200
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S1535947620338032
Volltext
Verfasserangaben:Nurhan Özlü, Flavio Monigatti, Bernhard Y. Renard, Christine M. Field, Hanno Steen, Timothy J. Mitchison, and Judith J. Steen
Beschreibung
Zusammenfassung:The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. Aurora-B binding partners switched dramatically between M phase to C phase, and we identified several novel C phase-selective Aurora-B binding partners including PRC1, KIF4, and anaphase-promoting complex/cyclosome. Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. Concretely, we report a central role for Aurora-B in regulating the C phase cytoskeleton.
Beschreibung:Online verfügbar 28 September 2009, Version des Artikels 4 January 2021
Gesehen am 21.06.2023
This work was supported, in whole or in part, by National Institutes of Health Grant GM 23928 (to T. J. M.). This work was also supported by a junior faculty development award from Children’s Hospital Boston and Harvard Medical School (to J. J. S.)
Beschreibung:Online Resource
ISSN:1535-9484
DOI:10.1074/mcp.M900308-MCP200

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