Cover Image

Role of TMEM100 in mechanically insensitive nociceptor un-silencing

Mechanically silent nociceptors are sensory afferents that are insensitive to noxious mechanical stimuli under normal conditions but become sensitized to such stimuli during inflammation. Using RNA-sequencing and quantitative RT-PCR we demonstrate that inflammation upregulates the expression of the...

Full description

Saved in:
Bibliographic Details
Main Authors: Nees, Timo A. (Author) , Wang, Na (Author) , Adamek, Pavel (Author) , Zeitzschel, Nadja (Author) , Verkest, Clement (Author) , La Porta, Carmen (Author) , Meyer, Irina (Author) , Virnich, Julie (Author) , Balkaya, Selin (Author) , Prato, Vincenzo (Author) , Morelli, Chiara (Author) , Begay, Valerie (Author) , Lee, Young Jae (Author) , Tappe-Theodor, Anke (Author) , Lewin, Gary R. (Author) , Heppenstall, Paul A. (Author) , Taberner, Francisco J. (Author) , Lechner, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 05 April 2023
In: Nature Communications
Year: 2023, Volume: 14, Pages: 1-17
ISSN:2041-1723
DOI:10.1038/s41467-023-37602-w
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-023-37602-w
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-023-37602-w
Get full text
Author Notes:Timo A. Nees, Na Wang, Pavel Adamek, Nadja Zeitzschel, Clement Verkest, Carmen La Porta, Irina Schaefer, Julie Virnich, Selin Balkaya, Vincenzo Prato, Chiara Morelli, Valerie Begay, Young Jae Lee, Anke Tappe-Theodor, Gary R. Lewin, Paul A. Heppenstall, Francisco J. Taberner & Stefan G. Lechner
Description
Summary:Mechanically silent nociceptors are sensory afferents that are insensitive to noxious mechanical stimuli under normal conditions but become sensitized to such stimuli during inflammation. Using RNA-sequencing and quantitative RT-PCR we demonstrate that inflammation upregulates the expression of the transmembrane protein TMEM100 in silent nociceptors and electrophysiology revealed that over-expression of TMEM100 is required and sufficient to un-silence silent nociceptors in mice. Moreover, we show that mice lacking TMEM100 do not develop secondary mechanical hypersensitivity - i.e., pain hypersensitivity that spreads beyond the site of inflammation - during knee joint inflammation and that AAV-mediated overexpression of TMEM100 in articular afferents in the absence of inflammation is sufficient to induce mechanical hypersensitivity in remote skin regions without causing knee joint pain. Thus, our work identifies TMEM100 as a key regulator of silent nociceptor un-silencing and reveals a physiological role for this hitherto enigmatic afferent subclass in triggering spatially remote secondary mechanical hypersensitivity during inflammation.
Item Description:Gesehen am 23.06.2023
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-023-37602-w

Similar Items