Cover Image

An unbiased genome-wide analysis of zinc-finger nuclease specificity

Zinc-finger nucleases allow targeted genetic modification at loci chosen by the investigator, but the extent of their off-target activity—which could be toxic to cells—has not been evaluated experimentally on a genome-wide scale. Gabriel et al. document the off- and on-target activity of zinc-finger...

Full description

Saved in:
Bibliographic Details
Main Authors: Gabriel, Richard (Author) , Lombardo, Angelo (Author) , Arens, Anne (Author) , Miller, Jeffrey C. (Author) , Genovese, Pietro (Author) , Käppel, Christine (Author) , Nowrouzi, Ali (Author) , Bartholomä, Cynthia C. (Author) , Wang, Jianbin (Author) , Friedman, Geoffrey (Author) , Holmes, Michael C. (Author) , Gregory, Philip D. (Author) , Glimm, Hanno (Author) , Schmidt, Manfred (Author) , Naldini, Luigi (Author) , Kalle, Christof von (Author)
Format: Article (Journal)
Language:English
Published: 7 August 2011
In: Nature biotechnology
Year: 2011, Volume: 29, Issue: 9, Pages: 816-823
ISSN:1546-1696
DOI:10.1038/nbt.1948
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nbt.1948
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nbt.1948
Get full text
Author Notes:Richard Gabriel, Angelo Lombardo, Anne Arens, Jeffrey C. Miller, Pietro Genovese, Christine Kaeppel, Ali Nowrouzi, Cynthia C. Bartholomae, Jianbin Wang, Geoffrey Friedman, Michael C. Holmes, Philip D. Gregory, Hanno Glimm, Manfred Schmidt, Luigi Naldini & Christof von Kalle
Description
Summary:Zinc-finger nucleases allow targeted genetic modification at loci chosen by the investigator, but the extent of their off-target activity—which could be toxic to cells—has not been evaluated experimentally on a genome-wide scale. Gabriel et al. document the off- and on-target activity of zinc-finger nucleases using lentiviral vectors to tag integration sites.
Item Description:Gesehen am 05.07.2023
Physical Description:Online Resource
ISSN:1546-1696
DOI:10.1038/nbt.1948

Similar Items