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An unbiased genome-wide analysis of zinc-finger nuclease specificity

Zinc-finger nucleases allow targeted genetic modification at loci chosen by the investigator, but the extent of their off-target activity—which could be toxic to cells—has not been evaluated experimentally on a genome-wide scale. Gabriel et al. document the off- and on-target activity of zinc-finger...

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Autori principali: Gabriel, Richard (Autore) , Lombardo, Angelo (Autore) , Arens, Anne (Autore) , Miller, Jeffrey C. (Autore) , Genovese, Pietro (Autore) , Käppel, Christine (Autore) , Nowrouzi, Ali (Autore) , Bartholomä, Cynthia C. (Autore) , Wang, Jianbin (Autore) , Friedman, Geoffrey (Autore) , Holmes, Michael C. (Autore) , Gregory, Philip D. (Autore) , Glimm, Hanno (Autore) , Schmidt, Manfred (Autore) , Naldini, Luigi (Autore) , Kalle, Christof von (Autore)
Natura: Article (Journal)
Lingua:inglese
Pubblicazione: 7 August 2011
In: Nature biotechnology
Year: 2011, Volume: 29, Fascicolo: 9, Pages: 816-823
ISSN:1546-1696
DOI:10.1038/nbt.1948
Accesso online:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/nbt.1948
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/nbt.1948
Testo
Note sull'autore:Richard Gabriel, Angelo Lombardo, Anne Arens, Jeffrey C. Miller, Pietro Genovese, Christine Kaeppel, Ali Nowrouzi, Cynthia C. Bartholomae, Jianbin Wang, Geoffrey Friedman, Michael C. Holmes, Philip D. Gregory, Hanno Glimm, Manfred Schmidt, Luigi Naldini & Christof von Kalle
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Riassunto:Zinc-finger nucleases allow targeted genetic modification at loci chosen by the investigator, but the extent of their off-target activity—which could be toxic to cells—has not been evaluated experimentally on a genome-wide scale. Gabriel et al. document the off- and on-target activity of zinc-finger nucleases using lentiviral vectors to tag integration sites.
Descrizione del documento:Gesehen am 05.07.2023
Descrizione fisica:Online Resource
ISSN:1546-1696
DOI:10.1038/nbt.1948

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