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Integrated proteome and acetylome analyses unveil protein features of gestational diabetes mellitus and preeclampsia

Gestational diabetes mellitus (GDM) and preeclampsia (PE) are associated with maternal and infant health. Although the pathogenesis of PE and GDM remains controversial, oxidative stress is involved in the underlying pathology of GDM and PE. Protein lysine acetylation (Kac) plays an important regulat...

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Main Authors: Hu, Biying (Author) , Li, Dandan (Author) , Tang, Donge (Author) , Shangguan, Yu (Author) , Cao, Yuzhi (Author) , Guo, Ruonan (Author) , Luan, Shaodong (Author) , Yun, Chen (Author) , Morgera, Stanislao (Author) , Hocher, Berthold (Author) , Krämer, Bernhard (Author) , Wang, Yinglan (Author) , Yin, Lianghong (Author) , Dai, Yong (Author)
Format: Article (Journal)
Language:English
Published: 12 September 2022
In: Proteomics
Year: 2022, Volume: 22, Issue: 22, Pages: 1-13
ISSN:1615-9861
DOI:10.1002/pmic.202200124
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/pmic.202200124
Verlag, lizenzpflichtig, Volltext: http://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.202200124
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Author Notes:Biying Hu, Dandan Li, Donge Tang, Yu Shangguan, Yuzhi Cao, Ruonan Guo, Shaodong Luan, Chen Yun, Stanislao Morgera, Berthold Hocher, Bernhard K. Krämer, Yinglan Wang, Lianghong Yin, Yong Dai
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Summary:Gestational diabetes mellitus (GDM) and preeclampsia (PE) are associated with maternal and infant health. Although the pathogenesis of PE and GDM remains controversial, oxidative stress is involved in the underlying pathology of GDM and PE. Protein lysine acetylation (Kac) plays an important regulatory role in biological processes. There is little data regarding the association of the maternal acetylome with GDM and PE. This study aimed to assess the potential value of the proteome and acetylome for GDM and PE. In our study, we included placental tissues from healthy individuals (n = 6), GDM patients (n = 6), and PE patients (n = 6) to perform 4D-label free quantification proteomics analysis and PRM analysis. We identified 22 significantly regulated proteins and 192 significantly regulated acetylated proteins between the GDM and PE groups. Furthermore, 192 significantly regulated acetylated proteins were mainly enriched in endoplasmic reticulum stress (ERS) and ferroptosis pathways. Seventeen acetylated sites in these two pathways were verified by PRM analysis. Our comprehensive analysis revealed key features of GDM/PE-significantly regulated acetylated proteins in the placentas from GDM and PE. The results of signaling pathway analysis focused on ERS and ferroptosis. These findings may help explore the underlying pathology, new biomarkers, and therapeutic targets of GDM and PE.
Item Description:Gesehen am 17.07.2023
Physical Description:Online Resource
ISSN:1615-9861
DOI:10.1002/pmic.202200124

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