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Triplet-encoded prebiotic RNA aminoacylation

The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzym...

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Bibliographic Details
Main Authors: Su, Meng (Author) , Schmitt, Christian (Author) , Liu, Ziwei (Author) , Roberts, Samuel J. (Author) , Liu, Kim C. (Author) , Röder, Konstantin (Author) , Jäschke, Andres (Author) , Wales, David J. (Author) , Sutherland, John D. (Author)
Format: Article (Journal)
Language:English
Published: July 26, 2023
In: Journal of the American Chemical Society
Year: 2023, Volume: 145, Issue: 29, Pages: 15971-15980
ISSN:1520-5126
DOI:10.1021/jacs.3c03931
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1021/jacs.3c03931
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Author Notes:Meng Su, Christian Schmitt, Ziwei Liu, Samuel J. Roberts, Kim C. Liu, Konstantin Röder, Andres Jäschke, David J. Wales, and John D. Sutherland
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Summary:The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzyme-free, sequence-dependent, chemoselective aminoacylation of RNA. We investigated two potentially prebiotic routes to aminoacyl-tRNA acceptor stem-overhang mimics and analyzed those oligonucleotides undergoing the most efficient aminoacylation. Overhang sequences do not significantly influence the chemoselectivity of aminoacylation by either route. For aminoacyl-transfer from a mixed anhydride donor strand, the chemoselectivity and stereoselectivity of aminoacylation depend on the terminal three base pairs of the stem. The results support early suggestions of a second genetic code in the acceptor stem.
Item Description:Online verfügbar: 12. Juli 2023
Gesehen am 21.08.2023
Physical Description:Online Resource
ISSN:1520-5126
DOI:10.1021/jacs.3c03931

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