Triplet-encoded prebiotic RNA aminoacylation
The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzym...
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| Hauptverfasser: | , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
July 26, 2023
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| In: |
Journal of the American Chemical Society
Year: 2023, Jahrgang: 145, Heft: 29, Pages: 15971-15980 |
| ISSN: | 1520-5126 |
| DOI: | 10.1021/jacs.3c03931 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: https://doi.org/10.1021/jacs.3c03931
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| Verfasserangaben: | Meng Su, Christian Schmitt, Ziwei Liu, Samuel J. Roberts, Kim C. Liu, Konstantin Röder, Andres Jäschke, David J. Wales, and John D. Sutherland |
| Zusammenfassung: | The encoding step of translation involves attachment of amino acids to cognate tRNAs by aminoacyl-tRNA synthetases, themselves the product of coded peptide synthesis. So, the question arises─before these enzymes evolved, how were primordial tRNAs selectively aminoacylated? Here, we demonstrate enzyme-free, sequence-dependent, chemoselective aminoacylation of RNA. We investigated two potentially prebiotic routes to aminoacyl-tRNA acceptor stem-overhang mimics and analyzed those oligonucleotides undergoing the most efficient aminoacylation. Overhang sequences do not significantly influence the chemoselectivity of aminoacylation by either route. For aminoacyl-transfer from a mixed anhydride donor strand, the chemoselectivity and stereoselectivity of aminoacylation depend on the terminal three base pairs of the stem. The results support early suggestions of a second genetic code in the acceptor stem. |
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| Beschreibung: | Online verfügbar: 12. Juli 2023 Gesehen am 21.08.2023 |
| Beschreibung: | Online Resource |
| ISSN: | 1520-5126 |
| DOI: | 10.1021/jacs.3c03931 |