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Early SARS-CoV-2 infection: platelet-neutrophil complexes and platelet function

Background - Conflicting results have been reported on platelet activity ex vivo and responsiveness in vitro among patients with COVID-19 with or without thromboembolic complications. - Objectives - To assess platelet reactivity in patients with moderate disease at early stages of COVID-19. - Method...

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Main Authors: Rieder, Marina (Author) , Baldus, Niklas (Author) , Stallmann, Daniela (Author) , Jeserich, Maren (Author) , Goller, Isabella (Author) , Wirth, Luisa (Author) , Pollmeier, Luisa (Author) , Hofmann, Maike (Author) , Bode, Christoph (Author) , Busch, Hans-Jörg (Author) , Schmid, Bonaventura (Author) , Gauchel, Nadine (Author) , Scharf, Rüdiger Eberhard (Author) , Dürschmied, Daniel (Author) , Lother, Achim (Author) , Krauel, Krystin (Author)
Format: Article (Journal)
Language:English
Published: January 2023
In: Research and practice in thrombosis and haemostasis
Year: 2023, Volume: 7, Issue: 1, Pages: 1-12
ISSN:2475-0379
DOI:10.1016/j.rpth.2022.100025
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.rpth.2022.100025
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S2475037922022762
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Author Notes:Marina Rieder, Niklas Baldus, Daniela Stallmann, Maren Jeserich, Isabella Goller, Luisa Wirth, Luisa Pollmeier, Maike Hofmann, Christoph Bode, Hans-Joerg Busch, Bonaventura Schmid, Nadine Gauchel, Rüdiger E. Scharf, Daniel Duerschmied, Achim Lother, Krystin Krauel
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Summary:Background - Conflicting results have been reported on platelet activity ex vivo and responsiveness in vitro among patients with COVID-19 with or without thromboembolic complications. - Objectives - To assess platelet reactivity in patients with moderate disease at early stages of COVID-19. - Methods - We performed a prospective, descriptive analysis of 100 consecutive patients presenting with suspected SARS-CoV-2 infection at University Medical Center Freiburg during the first or second wave of the pandemic. Following polymerase chain reaction testing and compliance with study inclusion criteria, 20 SARS-CoV-2-positive and 55 SARS-CoV-2-negative patients (serving as patient controls) were enrolled. In addition, 15 healthy subjects were included. Platelet reactivity was assessed using whole-blood impedance aggregometry and flow cytometry in response to various agonists. - Results - Platelet aggregation was significantly impaired in the patients with COVID-19 compared with that in the patient controls or healthy subjects. The reduced platelet responsiveness in the patients with COVID-19 was associated with impaired activation of GPIIb/IIIa (αIIbβ3). In contrast, low expression of P-selectin at baseline and intact secretion upon stimulation in vitro suggest that no preactivation in vivo, leading to “exhausted” platelets, had occurred. The proportion of circulating platelet-neutrophil complexes was significantly higher in the patients with COVID-19 (mean ± SD, 41% ± 13%) than in the patient controls (18% ± 7%; 95% CI, 11.1-34.1; P = .0002) or healthy subjects (17% ± 4%; 95% CI, 13.8-33.8; P < .0001). An analysis of neutrophil adhesion receptors revealed upregulation of CD11b (α-subunit of αMβ2) and CD66b (CEACAM8) but not of CD162 (PSGL-1) in the patients with COVID-19. - Conclusion - Despite reduced platelet responsiveness, platelet-neutrophil complexes are increased at early stages of moderate disease. Thus, this cellular interaction may occur during COVID-19 without preceding platelet activation.
Item Description:Online verfügbar: 23. Dezember 2022, Artikelversion: 11. März 2023
Gesehen am 22.08.2023
Physical Description:Online Resource
ISSN:2475-0379
DOI:10.1016/j.rpth.2022.100025

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