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Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces

Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes...

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Bibliographic Details
Main Authors: Beer, Alex G. F. de (Author) , Cavalcanti-Adam, Elisabetta A. (Author) , Majer, Günter (Author) , Lopez-García, M. (Author) , Kessler, H. (Author) , Spatz, Joachim P. (Author)
Format: Article (Journal)
Language:English
Published: 12 May 2010
In: Physical review. E, Statistical, nonlinear, and soft matter physics
Year: 2010, Volume: 81, Issue: 5, Pages: 1-7
ISSN:1550-2376
DOI:10.1103/PhysRevE.81.051914
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1103/PhysRevE.81.051914
Verlag, lizenzpflichtig, Volltext: https://link.aps.org/doi/10.1103/PhysRevE.81.051914
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Author Notes:Alex G.F. de Beer, E. Ada Cavalcanti-Adam, Günter Majer, M. Lopez-García, H. Kessler, and Joachim P. Spatz
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Summary:Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes is a decreased ability to form mature focal adhesions when lateral ligand distances exceed 76 nm. These changes are usually examined in the context of protein signaling and protein interactions. We show a physical explanation based on the balance between the forces acting on individual ligand connections and the reaction kinetics of those ligands. We propose three stability regimes for focal adhesions as a function of ligand spacing and applied stress: a stable regime, an unstable regime in which a large fraction of unbound protein causes adhesion disintegration, and a regime in which the applied force is too high to form an adhesion structure.
Item Description:Gesehen am 04.09.2023
Physical Description:Online Resource
ISSN:1550-2376
DOI:10.1103/PhysRevE.81.051914

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