Buchumschlag

Force-induced destabilization of focal adhesions at defined integrin spacings on nanostructured surfaces

Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Beer, Alex G. F. de (VerfasserIn) , Cavalcanti-Adam, Elisabetta A. (VerfasserIn) , Majer, Günter (VerfasserIn) , Lopez-García, M. (VerfasserIn) , Kessler, H. (VerfasserIn) , Spatz, Joachim P. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 12 May 2010
In: Physical review. E, Statistical, nonlinear, and soft matter physics
Year: 2010, Jahrgang: 81, Heft: 5, Pages: 1-7
ISSN:1550-2376
DOI:10.1103/PhysRevE.81.051914
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1103/PhysRevE.81.051914
Verlag, lizenzpflichtig, Volltext: https://link.aps.org/doi/10.1103/PhysRevE.81.051914
Volltext
Verfasserangaben:Alex G.F. de Beer, E. Ada Cavalcanti-Adam, Günter Majer, M. Lopez-García, H. Kessler, and Joachim P. Spatz
Beschreibung
Zusammenfassung:Focal adhesions are the anchoring points of cells to surfaces and are responsible for a large number of surface sensing processes. Nanopatterning studies have shown physiological changes in fibroblasts as a result of decreasing density of external binding ligands. The most striking of these changes is a decreased ability to form mature focal adhesions when lateral ligand distances exceed 76 nm. These changes are usually examined in the context of protein signaling and protein interactions. We show a physical explanation based on the balance between the forces acting on individual ligand connections and the reaction kinetics of those ligands. We propose three stability regimes for focal adhesions as a function of ligand spacing and applied stress: a stable regime, an unstable regime in which a large fraction of unbound protein causes adhesion disintegration, and a regime in which the applied force is too high to form an adhesion structure.
Beschreibung:Gesehen am 04.09.2023
Beschreibung:Online Resource
ISSN:1550-2376
DOI:10.1103/PhysRevE.81.051914

Ähnliche Einträge