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Continuous photobleaching to study the growth modes of focal adhesions

We combine total internal reflection fluorescence microscopy with continuous photobleaching to study the growth mode of focal adhesions in rat embryonic fibroblasts (REF-52). We measured GFP-labelled β3 integrin, which exhibits a diffusion rate of 10-14-10-13 m2/s when it is not bound to a focal adh...

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Bibliographic Details
Main Authors: Beer, Alex G. F. de (Author) , Majer, Günter (Author) , Roke, Sylvie (Author) , Spatz, Joachim P. (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: Journal of adhesion science and technology
Year: 2010, Volume: 24, Issue: 13-14, Pages: 2323-2334
ISSN:1568-5616
DOI:10.1163/016942410X508046
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1163/016942410X508046
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Author Notes:Alex G.F. de Beer, Günter Majer, Sylvie Roke, Joachim P. Spatz
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Summary:We combine total internal reflection fluorescence microscopy with continuous photobleaching to study the growth mode of focal adhesions in rat embryonic fibroblasts (REF-52). We measured GFP-labelled β3 integrin, which exhibits a diffusion rate of 10-14-10-13 m2/s when it is not bound to a focal adhesion. We show that exchange of β3 integrin between focal adhesions and the surrounding membrane occurs only at the edges of the focal adhesion. We, therefore, conclude that focal adhesions show an edge-on/edge-off growth, in contrast to the bulk-on/bulk-off growth mode that is assumed in many theoretical treatments.
Item Description:Online veröffentlicht: 02 April 2012
Gesehen am 04.09.2023
Physical Description:Online Resource
ISSN:1568-5616
DOI:10.1163/016942410X508046

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