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Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 Binding

The tails of histone proteins are central players for all chromatin-mediated processes. Whereas the N-terminal histone tails have been studied extensively, little is known about the function of the H2A C-terminus. Here, we show that the H2A C-terminal tail plays a pivotal role in regulating chromati...

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Main Authors: Vogler, Christine (Author) , Huber, Claudia (Author) , Waldmann, Tanja (Author) , Ettig, Ramona (Author) , Braun, Lora (Author) , Izzo, Annalisa (Author) , Daujat, Sylvain (Author) , Chassignet, Isabelle (Author) , Lopez-Contreras, Andres Joaquin (Author) , Fernandez-Capetillo, Oscar (Author) , Dundr, Miroslav (Author) , Rippe, Karsten (Author) , Längst, Gernot (Author) , Schneider, Robert (Author)
Format: Article (Journal)
Language:English
Published: December 9, 2010
In: PLoS Genetics
Year: 2010, Volume: 6, Issue: 12, Pages: 1-12
ISSN:1553-7404
DOI:10.1371/journal.pgen.1001234
Online Access:Resolving-System, kostenfrei, Volltext: https://doi.org/10.1371/journal.pgen.1001234
Verlag, kostenfrei, Volltext: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1001234
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Author Notes:Christine Vogler, Claudia Huber, Tanja Waldmann, Ramona Ettig, Lora Braun, Annalisa Izzo, Sylvain Daujat, Isabelle Chassignet, Andres Joaquin Lopez-Contreras, Oscar Fernandez-Capetillo, Miroslav Dundr, Karsten Rippe, Gernot Längst, Robert Schneider
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Summary:The tails of histone proteins are central players for all chromatin-mediated processes. Whereas the N-terminal histone tails have been studied extensively, little is known about the function of the H2A C-terminus. Here, we show that the H2A C-terminal tail plays a pivotal role in regulating chromatin structure and dynamics. We find that cells expressing C-terminally truncated H2A show increased stress sensitivity. Moreover, both the complete and the partial deletion of the tail result in increased histone exchange kinetics and nucleosome mobility in vivo and in vitro. Importantly, our experiments reveal that the H2A C-terminus is required for efficient nucleosome translocation by ISWI-type chromatin remodelers and acts as a novel recognition module for linker histone H1. Thus, we suggest that the H2A C-terminal tail has a bipartite function: stabilisation of the nucleosomal core particle, as well as mediation of the protein interactions that control chromatin dynamics and conformation.
Item Description:Gesehen am 12.09.2023
Physical Description:Online Resource
ISSN:1553-7404
DOI:10.1371/journal.pgen.1001234

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