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Serotonin 3A receptor subtype as an early and protracted marker of cortical interneuron subpopulations

To identify neocortical neurons expressing the type 3 serotonergic receptor, here we used transgenic mice expressing the enhanced green fluorescent protein (GFP) under the control of the 5-HT3A promoter (5-HT3A:GFP mice). By means of whole-cell patch-clamp recordings, biocytin labeling, and single-c...

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Main Authors: Vucurovic, Ksenija (Author) , Gallopin, Thierry (Author) , Ferezou, Isabelle (Author) , Rancillac, Armelle (Author) , Chameau, Pascal (Author) , van Hooft, Johannes A. (Author) , Geoffroy, Hélène (Author) , Monyer, Hannah (Author) , Rossier, Jean (Author) , Vitalis, Tania (Author)
Format: Article (Journal)
Language:English
Published: January 18, 2010
In: Cerebral cortex
Year: 2010, Volume: 20, Issue: 10, Pages: 2333-2347
ISSN:1460-2199
DOI:10.1093/cercor/bhp310
Online Access:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1093/cercor/bhp310
Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/cercor/article/20/10/2333/317038
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Author Notes:Ksenija Vucurovic, Thierry Gallopin, Isabelle Ferezou, Armelle Rancillac, Pascal Chameau, Johannes A. van Hooft, Hélène Geoffroy, Hannah Monyer, Jean Rossier and Tania Vitalis
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Summary:To identify neocortical neurons expressing the type 3 serotonergic receptor, here we used transgenic mice expressing the enhanced green fluorescent protein (GFP) under the control of the 5-HT3A promoter (5-HT3A:GFP mice). By means of whole-cell patch-clamp recordings, biocytin labeling, and single-cell reversed-transcriptase polymerase chain reaction on acute brain slices of 5-HT3A:GFP mice, we identified 2 populations of 5-HT3A-expressing interneurons within the somatosensory cortex. The first population was characterized by the frequent expression of the vasoactive intestinal peptide and a typical bipolar/bitufted morphology, whereas the second population expressed predominantly the neuropeptide Y and exhibited more complex dendritic arborizations. Most interneurons of this second group appeared very similar to neurogliaform cells according to their electrophysiological, molecular, and morphological properties. The combination of 5-bromo-2-deoxyuridine injections with 5-HT3A mRNA detection showed that cortical 5-HT3A interneurons are generated around embryonic day 14.5. Although at this stage the 5-HT3A receptor subunit is expressed in both the caudal ganglionic eminence and the entopeduncular area, homochronic in utero grafts experiments revealed that cortical 5-HT3A interneurons are mainly generated in the caudal ganglionic eminence. This protracted expression of the 5-HT3A subunit allowed us to study specific cortical interneuron populations from their birth to their final functional phenotype.
Item Description:Gesehen am 19.09.2023
Physical Description:Online Resource
ISSN:1460-2199
DOI:10.1093/cercor/bhp310

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