Buchumschlag

Serotonin 3A receptor subtype as an early and protracted marker of cortical interneuron subpopulations

To identify neocortical neurons expressing the type 3 serotonergic receptor, here we used transgenic mice expressing the enhanced green fluorescent protein (GFP) under the control of the 5-HT3A promoter (5-HT3A:GFP mice). By means of whole-cell patch-clamp recordings, biocytin labeling, and single-c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Vucurovic, Ksenija (VerfasserIn) , Gallopin, Thierry (VerfasserIn) , Ferezou, Isabelle (VerfasserIn) , Rancillac, Armelle (VerfasserIn) , Chameau, Pascal (VerfasserIn) , van Hooft, Johannes A. (VerfasserIn) , Geoffroy, Hélène (VerfasserIn) , Monyer, Hannah (VerfasserIn) , Rossier, Jean (VerfasserIn) , Vitalis, Tania (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: January 18, 2010
In: Cerebral cortex
Year: 2010, Jahrgang: 20, Heft: 10, Pages: 2333-2347
ISSN:1460-2199
DOI:10.1093/cercor/bhp310
Online-Zugang:Resolving-System, lizenzpflichtig, Volltext: https://doi.org/10.1093/cercor/bhp310
Verlag, lizenzpflichtig, Volltext: https://academic.oup.com/cercor/article/20/10/2333/317038
Volltext
Verfasserangaben:Ksenija Vucurovic, Thierry Gallopin, Isabelle Ferezou, Armelle Rancillac, Pascal Chameau, Johannes A. van Hooft, Hélène Geoffroy, Hannah Monyer, Jean Rossier and Tania Vitalis
Beschreibung
Zusammenfassung:To identify neocortical neurons expressing the type 3 serotonergic receptor, here we used transgenic mice expressing the enhanced green fluorescent protein (GFP) under the control of the 5-HT3A promoter (5-HT3A:GFP mice). By means of whole-cell patch-clamp recordings, biocytin labeling, and single-cell reversed-transcriptase polymerase chain reaction on acute brain slices of 5-HT3A:GFP mice, we identified 2 populations of 5-HT3A-expressing interneurons within the somatosensory cortex. The first population was characterized by the frequent expression of the vasoactive intestinal peptide and a typical bipolar/bitufted morphology, whereas the second population expressed predominantly the neuropeptide Y and exhibited more complex dendritic arborizations. Most interneurons of this second group appeared very similar to neurogliaform cells according to their electrophysiological, molecular, and morphological properties. The combination of 5-bromo-2-deoxyuridine injections with 5-HT3A mRNA detection showed that cortical 5-HT3A interneurons are generated around embryonic day 14.5. Although at this stage the 5-HT3A receptor subunit is expressed in both the caudal ganglionic eminence and the entopeduncular area, homochronic in utero grafts experiments revealed that cortical 5-HT3A interneurons are mainly generated in the caudal ganglionic eminence. This protracted expression of the 5-HT3A subunit allowed us to study specific cortical interneuron populations from their birth to their final functional phenotype.
Beschreibung:Gesehen am 19.09.2023
Beschreibung:Online Resource
ISSN:1460-2199
DOI:10.1093/cercor/bhp310

Ähnliche Einträge