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A fluorometric assay to determine labile copper(II) ions in serum

Labile copper(II) ions (Cu2+) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson’s disease (WD) or neurological disorders. S...

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Main Authors: Maares, Maria (Author) , Haupt, Alessia (Author) , Schüßler, Christoph (Author) , Kulike-Koczula, Marcel (Author) , Hackler, Julian (Author) , Keil, Claudia (Author) , Mohr, Isabelle (Author) , Schomburg, Lutz (Author) , Süssmuth, Roderich D. (Author) , Zischka, Hans (Author) , Merle, Uta (Author) , Haase, Hajo (Author)
Format: Article (Journal)
Language:English
Published: 07 August 2023
In: Scientific reports
Year: 2023, Volume: 13, Pages: 1-10
ISSN:2045-2322
DOI:10.1038/s41598-023-39841-9
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41598-023-39841-9
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41598-023-39841-9
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Author Notes:Maria Maares, Alessia Haupt, Christoph Schüßler, Marcel Kulike-Koczula, Julian Hackler, Claudia Keil, Isabelle Mohr, Lutz Schomburg, Roderich D. Süssmuth, Hans Zischka, Uta Merle & Hajo Haase
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Summary:Labile copper(II) ions (Cu2+) in serum are considered to be readily available for cellular uptake and to constitute the biologically active Cu2+ species in the blood. It might also be suitable to reflect copper dyshomeostasis during diseases such as Wilson’s disease (WD) or neurological disorders. So far, no direct quantification method has been described to determine this small Cu2+ subset. This study introduces a fluorometric high throughput assay using the novel Cu2+ binding fluoresceine-peptide sensor FP4 (Kd of the Cu2+-FP4-complex 0.38 pM) to determine labile Cu2+ in human and rat serum. ...
Item Description:Gesehen am 12.12.2023
Physical Description:Online Resource
ISSN:2045-2322
DOI:10.1038/s41598-023-39841-9

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