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Opsonization-independent antigen-specific recognition by myeloid phagocytes expressing monoclonal antibodies

This report demonstrates a novel class of innate immune cells designated “variable immunoreceptor-expressing myeloids” (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of...

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Hauptverfasser: Neumaier, Michael (VerfasserIn) , Giesler, Sophie (VerfasserIn) , Ast, Volker (VerfasserIn) , Römer, Mathis (VerfasserIn) , Voß, Timo-Daniel (VerfasserIn) , Reinz, Eileen (VerfasserIn) , Costina, Victor (VerfasserIn) , Schmelz, Martin (VerfasserIn) , Nürnberg, Elina (VerfasserIn) , Nittka, Stefanie (VerfasserIn) , Leppä, Aino-Maija (VerfasserIn) , Rudolf, Rüdiger (VerfasserIn) , Trumpp, Andreas (VerfasserIn) , Fuchs, Tina (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: Sep 2023
In: Science advances
Year: 2023, Jahrgang: 9, Heft: 35, Pages: 1-12
ISSN:2375-2548
DOI:10.1126/sciadv.adg1812
Online-Zugang:Resolving-System, kostenfrei: https://doi.org/10.1126/sciadv.adg1812
Volltext
Verfasserangaben:Michael Neumaier, Sophie Giesler, Volker Ast, Mathis Roemer, Timo-Daniel Voß, Eileen Reinz, Victor Costina, Martin Schmelz, Elina Nürnberg, Stefanie Nittka, Aino-Maija Leppä, Ruediger Rudolf, Andreas Trumpp, Tina Fuchs
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Zusammenfassung:This report demonstrates a novel class of innate immune cells designated “variable immunoreceptor-expressing myeloids” (VIREMs). Using single-cell transcriptomics and genome-wide epigenetic profiling, we establish that VIREMs are myeloid cells unrelated to lymphocytes. We visualize the phenotype of B-VIREMs that are capable of genetically recombining and expressing antibody genes, the exclusive hallmark function of B lymphocytes. These cells, designated B-VIREMs, display monoclonal antibody cell surface signatures and regularly circulate in the blood of healthy individuals. Single-cell data reveal clonal expansion of circulating B-VIREMs as a dynamic response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport to the cell surface. A first cloned B-VIREM-derived antibody (Vab1) specifically binds stomatin, a ubiquitous scaffold protein that is strictly expressed intracellularly, allowing Vab1-bearing macrophages to phagocytose cell debris without requiring prior opsonization. Our results suggest important antigen-specific tissue maintenance functionalities in these innate immune cells.
Beschreibung:Online veröffentlicht: 01. September 2023
Gesehen am 09.11.2023
Beschreibung:Online Resource
ISSN:2375-2548
DOI:10.1126/sciadv.adg1812

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