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A versatile system for the neuronal subtype specific expression of lentiviral vectors

Lentiviral expression vectors are powerful tools for gene therapy and long-term gene expression/repression in the mammalian brain. However, no specificity of transduction has been reported so far in the central nervous system. Here we have developed a novel system to achieve a neuronal subtype speci...

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Main Authors: Tolu, Stefania (Author) , Avale, M. Elena (Author) , Nakatani, Hiroko (Author) , Pons, Stephanie (Author) , Parnaudeau, Sebastien (Author) , Tronche, Francois (Author) , Vogt, Angelika (Author) , Monyer, Hannah (Author) , Vogel, Roland (Author) , Chaumont, Fabrice de (Author) , Olivo-Marín, Jean-Christophe (Author) , Changeux, Jean-Pierre (Author) , Maskos, Uwe (Author)
Format: Article (Journal)
Language:English
Published: 2010
In: The FASEB journal
Year: 2010, Volume: 24, Issue: 3, Pages: 723-730
ISSN:1530-6860
DOI:10.1096/fj.09-139790
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1096/fj.09-139790
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.09-139790
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Author Notes:Stefania Tolu, M. Elena Avale, Hiroko Nakatani, Stephanie Pons, Sebastien Parnaudeau, Francois Tronche, Angelika Vogt, Hannah Monyer, Roland Vogel, Fabrice de Chaumont, Jean-Christophe Olivo-Marín, Jean-Pierre Changeux, and Uwe Maskos
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Summary:Lentiviral expression vectors are powerful tools for gene therapy and long-term gene expression/repression in the mammalian brain. However, no specificity of transduction has been reported so far in the central nervous system. Here we have developed a novel system to achieve a neuronal subtype specific expression in either dopaminergic (DA) or GABAergic neurons. We employed a delivery strategy by which the transgene is not expressed until its activation by Cre recombinase. We successfully tested the system in vitro and then used this novel lentivector, containing loxP sites, in 2 different transgenic mouse lines expressing Cre either in DA or in GABAergic neurons. In both lines the reporter gene was detected exclusively in Cre-positive cells, demonstrating that with this experimental approach we were able to achieve completely specific expression of transgenes delivered by lentiviral vectors. This universal system can be applied to all neural subtypes making use of the growing number of specific Cre driver lines.—Tolu, S., Avale, M. E., Nakatani, H., Pons, S., Parnaudeau, S., Tronche, F., Vogt, A., Monyer, H., Vogel, R., de Chaumont, F., Olivo-Marin, J.-C., Changeux, J.-P., Maskos, U. A versatile system for the neuronal subtype specific expression of lentiviral vectors. FASEB J. 24, 723-730 (2010). www.fasebj.org
Item Description:Erstmals am 26 October 2009 online veröffentlicht
Gesehen am 28.11.2023
Physical Description:Online Resource
ISSN:1530-6860
DOI:10.1096/fj.09-139790

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