A new experimental model to study shrimp allergy
Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new exp...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
August 2023
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| In: |
Immunology letters
Year: 2023, Volume: 260, Pages: 73-80 |
| ISSN: | 1879-0542 |
| DOI: | 10.1016/j.imlet.2023.06.007 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.imlet.2023.06.007 Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0165247823001098 
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| Author Notes: | Ivanéia Valeriano Nunes, Camila Mattos Andrade, Priscila Valera Guerra, Mariana Ivo Khouri, Maria Poliana Leite Galantini, Robson Amaro Augusto da Silva, Eliana L. Faquim-Mauro, Leonardo Paiva Farias, Juliana de Souza Rebouças, Ana Maria Caetano Faria, Cláudia Ida Brodskyn |
| Summary: | Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new experimental model of shrimp allergy that could enable the evaluation of new prophylactic treatments. BALB/c mice were subcutaneously sensitized with 100 μg of shrimp proteins of Litopenaeus vannamei adsorbed in 1 mg of aluminum hydroxide on day 0, and a booster (100 µg of shrimp proteins only) on day 14. The oral challenge protocol was based on the addition of 5 mg/ml of shrimp proteins to water from day 21 to day 35. Analysis of shrimp extract content detected at least 4 of the major allergens reported to L. vannamei. In response to the sensitization, allergic mice showed significantly enhanced IL-4 and IL-10 production in restimulated cervical draining lymph node cells. High detection of serum anti-shrimp IgE and IgG1 suggested the development of allergies to shrimp while Passive Cutaneous Anaphylaxis assay revealed an IgE-mediated response. Immunoblotting analysis revealed that Allergic mice developed antibodies to multiple antigens present in the shrimp extract. These observations were supported by the detection of anti-shrimp IgA production in intestinal lavage samples and morphometric intestinal mucosal changes. Therefore, this experimental protocol can be a tool to evaluate prophylactic and therapeutic approaches. |
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| Item Description: | Gesehen am 28.11.2023 Online verfügbar: 13. Juni 2023, Version des Artikels: 5. Juli 2023 |
| Physical Description: | Online Resource |
| ISSN: | 1879-0542 |
| DOI: | 10.1016/j.imlet.2023.06.007 |