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A new experimental model to study shrimp allergy

Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new exp...

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Hauptverfasser: Valeriano Nunes, Ivanéia (VerfasserIn) , Andrade, Camila Mattos (VerfasserIn) , Guerra, Priscila Valera (VerfasserIn) , Khouri, Mariana Ivo (VerfasserIn) , Galantini, Maria Poliana Leite (VerfasserIn) , da Silva, Robson Amaro Augusto (VerfasserIn) , Faquim-Mauro, Eliana L. (VerfasserIn) , Farias, Leonardo Paiva (VerfasserIn) , Rebouças, Juliana de Souza (VerfasserIn) , Faria, Ana Maria Caetano (VerfasserIn) , Brodskyn, Cláudia Ida (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: August 2023
In: Immunology letters
Year: 2023, Jahrgang: 260, Pages: 73-80
ISSN:1879-0542
DOI:10.1016/j.imlet.2023.06.007
Online-Zugang:Verlag, kostenfrei, Volltext: https://doi.org/10.1016/j.imlet.2023.06.007
Verlag, kostenfrei, Volltext: https://www.sciencedirect.com/science/article/pii/S0165247823001098
Volltext
Verfasserangaben:Ivanéia Valeriano Nunes, Camila Mattos Andrade, Priscila Valera Guerra, Mariana Ivo Khouri, Maria Poliana Leite Galantini, Robson Amaro Augusto da Silva, Eliana L. Faquim-Mauro, Leonardo Paiva Farias, Juliana de Souza Rebouças, Ana Maria Caetano Faria, Cláudia Ida Brodskyn
Beschreibung
Zusammenfassung:Shrimp is among the most sensitizing food allergens and has been associated with many anaphylaxis reactions. However, there is still a shortage of studies that enable a systematic understanding of this disease and the investigation of new therapeutic approaches. This study aimed to develop a new experimental model of shrimp allergy that could enable the evaluation of new prophylactic treatments. BALB/c mice were subcutaneously sensitized with 100 μg of shrimp proteins of Litopenaeus vannamei adsorbed in 1 mg of aluminum hydroxide on day 0, and a booster (100 µg of shrimp proteins only) on day 14. The oral challenge protocol was based on the addition of 5 mg/ml of shrimp proteins to water from day 21 to day 35. Analysis of shrimp extract content detected at least 4 of the major allergens reported to L. vannamei. In response to the sensitization, allergic mice showed significantly enhanced IL-4 and IL-10 production in restimulated cervical draining lymph node cells. High detection of serum anti-shrimp IgE and IgG1 suggested the development of allergies to shrimp while Passive Cutaneous Anaphylaxis assay revealed an IgE-mediated response. Immunoblotting analysis revealed that Allergic mice developed antibodies to multiple antigens present in the shrimp extract. These observations were supported by the detection of anti-shrimp IgA production in intestinal lavage samples and morphometric intestinal mucosal changes. Therefore, this experimental protocol can be a tool to evaluate prophylactic and therapeutic approaches.
Beschreibung:Gesehen am 28.11.2023
Online verfügbar: 13. Juni 2023, Version des Artikels: 5. Juli 2023
Beschreibung:Online Resource
ISSN:1879-0542
DOI:10.1016/j.imlet.2023.06.007

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