Myosin V facilitates polarised E-cadherin secretion
E-cadherin has a fundamental role in epithelial tissues by providing cell-cell adhesion. Polarised E-cadherin exocytosis to the lateral plasma membrane is central for cell polarity and epithelial homeostasis. Loss of E-cadherin secretion compromises tissue integrity and is a prerequisite for metasta...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
July 2022
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| In: |
Traffic
Year: 2022, Volume: 23, Issue: 7, Pages: 374-390 |
| ISSN: | 1600-0854 |
| DOI: | 10.1111/tra.12846 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1111/tra.12846 Verlag, kostenfrei, Volltext: http://onlinelibrary.wiley.com/doi/abs/10.1111/tra.12846 
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| Author Notes: | Dajana Tanasic, Nicola Berns, Veit Riechmann |
| Summary: | E-cadherin has a fundamental role in epithelial tissues by providing cell-cell adhesion. Polarised E-cadherin exocytosis to the lateral plasma membrane is central for cell polarity and epithelial homeostasis. Loss of E-cadherin secretion compromises tissue integrity and is a prerequisite for metastasis. Despite this pivotal role of E-cadherin secretion, the transport mechanism is still unknown. Here we identify Myosin V as the motor for E-cadherin secretion. Our data reveal that Myosin V and F-actin are required for the formation of a continuous apicolateral E-cadherin belt, the zonula adherens. We show by live imaging how Myosin V transports E-cadherin vesicles to the plasma membrane, and distinguish two distinct transport tracks: an apical actin network leading to the zonula adherens and parallel actin bundles leading to the basal-most region of the lateral membrane. E-cadherin secretion starts in endosomes, where Rab11 and Sec15 recruit Myosin V for transport to the zonula adherens. We also shed light on the endosomal sorting of E-cadherin by showing how Rab7 and Snx16 cooperate in moving E-cadherin into the Rab11 compartment. Thus, our data help to understand how polarised E-cadherin secretion maintains epithelial architecture and prevents metastasis. |
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| Item Description: | First published: 16 May 2022 Gesehen am 19.12.2023 |
| Physical Description: | Online Resource |
| ISSN: | 1600-0854 |
| DOI: | 10.1111/tra.12846 |