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Generation of patient-derived pediatric pilocytic astrocytoma in-vitro models using SV40 large T: evaluation of a modeling workflow

Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-gra...

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Main Authors: Selt, Florian (Author) , El Damaty, Ahmed (Author) , Schuhmann, Martin U. (Author) , Sigaud, Romain (Author) , Ecker, Jonas (Author) , Sievers, Philipp (Author) , Kocher, Daniela (Author) , Herold-Mende, Christel (Author) , Oehme, Ina (Author) , Deimling, Andreas von (Author) , Pfister, Stefan (Author) , Sahm, Felix (Author) , Jones, David T. W. (Author) , Witt, Olaf (Author) , Milde, Till (Author)
Format: Article (Journal)
Language:English
Published: 24 November 2023
In: Journal of neuro-oncology
Year: 2023, Volume: 165, Issue: 3, Pages: 467-478
ISSN:1573-7373
DOI:10.1007/s11060-023-04500-6
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1007/s11060-023-04500-6
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Author Notes:Florian Selt, Ahmed El Damaty, Martin U. Schuhmann, Romain Sigaud, Jonas Ecker, Philipp Sievers, Daniela Kocher, Christel Herold-Mende, Ina Oehme, Andreas von Deimling, Stefan M. Pfister, Felix Sahm, David T.W. Jones, Olaf Witt, Till Milde
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Summary:Although pediatric low-grade gliomas (pLGG) are the most common pediatric brain tumors, patient-derived cell lines reflecting pLGG biology in culture are scarce. This also applies to the most common pLGG subtype pilocytic astrocytoma (PA). Conventional cell culture approaches adapted from higher-grade tumors fail in PA due to oncogene-induced senescence (OIS) driving tumor cells into arrest. Here, we describe a PA modeling workflow using the Simian Virus large T antigen (SV40-TAg) to circumvent OIS.
Item Description:Gesehen am 22.01.2024
Physical Description:Online Resource
ISSN:1573-7373
DOI:10.1007/s11060-023-04500-6

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