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Changes in neuropeptide large dense core vesicle trafficking dynamics contribute to adaptive responses to a systemic homeostatic challenge

Neuropeptides are packed into large dense core vesicles (LDCVs) that are transported from the soma out into their processes. Limited information exists regarding mechanisms regulating LDCV trafficking, particularly during challenges to bodily homeostasis. Addressing this gap, we used 2-photon imagin...

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Hauptverfasser: Kirchner, Matthew K. (VerfasserIn) , Althammer, Ferdinand (VerfasserIn) , Donaldson, Kevin J. (VerfasserIn) , Cox, Daniel N. (VerfasserIn) , Stern, Javier E. (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: 17 November 2023
In: iScience
Year: 2023, Jahrgang: 26, Heft: 11, Pages: [1], 1-15
ISSN:2589-0042
DOI:10.1016/j.isci.2023.108243
Online-Zugang:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/j.isci.2023.108243
Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S2589004223023209
Volltext
Verfasserangaben:Matthew K. Kirchner, Ferdinand Althammer, Kevin J. Donaldson, Daniel N. Cox, and Javier E. Stern
Beschreibung
Zusammenfassung:Neuropeptides are packed into large dense core vesicles (LDCVs) that are transported from the soma out into their processes. Limited information exists regarding mechanisms regulating LDCV trafficking, particularly during challenges to bodily homeostasis. Addressing this gap, we used 2-photon imaging in an ex vivo preparation to study LDCVs trafficking dynamics in vasopressin (VP) neurons, which traffic and release neuropeptide from their dendrites and axons. We report a dynamic bidirectional trafficking of VP-LDCVs with important differences in speed and directionality between axons and dendrites. Acute, short-lasting stimuli known to alter VP firing activity and axonal/dendritic release caused modest changes in VP-LDCVs trafficking dynamics. Conversely, chronic/sustained systemic osmotic challenges upregulated VP-LDCVs trafficking dynamic, with a larger effect in dendrites. These results support differential regulation of dendritic and axonal LDCV trafficking, and that changes in trafficking dynamics constitute a novel mechanism by which peptidergic neurons can efficiently adapt to conditions of increased hormonal demand.
Beschreibung:Gesehen am 19.02.2023
Beschreibung:Online Resource
ISSN:2589-0042
DOI:10.1016/j.isci.2023.108243

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