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Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels [data]

This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabric...

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Main Authors: Schwegler, Niklas (Author) , Gebert, Tanisha (Author) , Villiou, Maria (Author) , Colombo, Federico (Author) , Schamberger, Barbara (Author) , Selhuber-Unkel, Christine (Author) , Thomas, Franziska (Author) , Blasco, Eva (Author)
Format: Database Research Data
Language:English
Published: Heidelberg Universität 2024-02-16
DOI:10.11588/data/V2XXS8
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Online Access:Resolving-System, kostenfrei, Volltext: https://doi.org/10.11588/data/V2XXS8
Verlag, kostenfrei, Volltext: https://heidata.uni-heidelberg.de/dataset.xhtml?persistentId=doi:10.11588/data/V2XXS8
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Author Notes:Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, Eva Blasco
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Summary:This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments.
Item Description:Finanziert durch: Spotlight Project “Synthetic Immunology” of the Flagship Initiative Engineering Molecular Systems (FI EMS; funded by the BMBF and the Ministry of Science Baden-Württemberg); Excellence Cluster “3D Matter Made to Order” (funded by the DFG): EXC 2082/1 390761711; ERC Consolidator Grant PHOTOMECH: Grant no. 101001797; Volkswagen Foundation Initiative “Life?,”: Az. 96733
Gesehen am 21.02.2024
Physical Description:Online Resource
DOI:10.11588/data/V2XXS8

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