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Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels

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This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabric...

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Bibliographic Details
Main Authors: Schwegler, Niklas (Author) , Gebert, Tanisha (Author) , Villiou, Maria (Author) , Colombo, Federico (Author) , Schamberger, Barbara (Author) , Selhuber-Unkel, Christine (Author) , Thomas, Franziska (Author) , Blasco, Eva (Author)
Format: Article (Journal) Chapter/Article
Language:English
Published: 17 January 2024
Edition:Version 1
In: ChemRxiv
Year: 2024, Pages: 1-13
DOI:10.26434/chemrxiv-2024-f94sf
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.26434/chemrxiv-2024-f94sf
Verlag, lizenzpflichtig, Volltext: https://chemrxiv.org/engage/chemrxiv/article-details/65a63933e9ebbb4db943fa99
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Author Notes:Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, and Eva Blasco
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Summary:This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments.
Item Description:Gesehen am 21.02.2024
Physical Description:Online Resource
DOI:10.26434/chemrxiv-2024-f94sf

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