Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels
This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabric...
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| Hauptverfasser: | , , , , , , , |
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| Dokumenttyp: | Article (Journal) Kapitel/Artikel |
| Sprache: | Englisch |
| Veröffentlicht: |
17 January 2024
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| Ausgabe: | Version 1 |
| In: |
ChemRxiv
Year: 2024, Pages: 1-13 |
| DOI: | 10.26434/chemrxiv-2024-f94sf |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.26434/chemrxiv-2024-f94sf Verlag, lizenzpflichtig, Volltext: https://chemrxiv.org/engage/chemrxiv/article-details/65a63933e9ebbb4db943fa99 
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| Verfasserangaben: | Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, and Eva Blasco |
| Zusammenfassung: | This study introduces a straightforward method for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability and rapid and precise 3D fabrication. The printable cell-adhesive PEG-based material includes an RGD-containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of our system, we showcase the fabrication of cell-adhesive 2.5D and 3D structures, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments. |
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| Beschreibung: | Gesehen am 21.02.2024 |
| Beschreibung: | Online Resource |
| DOI: | 10.26434/chemrxiv-2024-f94sf |