Efficient single copy integration via homology-directed repair (scHDR) by 5′modification of large DNA donor fragments in mice
CRISPR/Cas-based approaches have largely replaced conventional gene targeting strategies. However, homology-directed repair (HDR) in the mouse genome is not very efficient, and precisely inserting longer sequences using HDR remains challenging given that donor constructs preferentially integrate as...
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| Hauptverfasser: | , , , , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
22 February 2023
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| In: |
Nucleic acids research
Year: 2023, Jahrgang: 51, Heft: 3, Pages: 1-14 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkac1150 |
| Online-Zugang: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1093/nar/gkac1150 |
| Verfasserangaben: | Rebekka Medert, Thomas Thumberger, Tinatini Tavhelidse-Suck, Tobias Hub, Tanja Kellner, Yoko Oguchi, Sascha Dlugosz, Frank Zimmermann, Joachim Wittbrodt and Marc Freichel |
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Efficient single copy integration via homology-directed repair (scHDR) by 5’modification of large DNA donor fragments in mice
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