Evaluation of bioprinted autologous cartilage grafts in an immunocompetent rabbit model
The gold standard of auricular reconstruction involves manual graft assembly from autologous costal cartilage. The intervention may require multiple surgical procedures and lead to donor-site morbidity, while the outcome is highly dependent on individual surgical skills. A tissue engineering approac...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
04 March 2024
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| In: |
Advanced therapeutics
Year: 2024, Pages: 1-24 |
| ISSN: | 2366-3987 |
| DOI: | 10.1002/adtp.202300441 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1002/adtp.202300441 Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/adtp.202300441 
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| Author Notes: | David Gvaramia, Philipp Fisch, Killian Flégeau, Lena Huber, Johann Kern, Yvonne Jakob, Daniela Hirsch, Nicole Rotter |
| Summary: | The gold standard of auricular reconstruction involves manual graft assembly from autologous costal cartilage. The intervention may require multiple surgical procedures and lead to donor-site morbidity, while the outcome is highly dependent on individual surgical skills. A tissue engineering approach provides the means to produce cartilage grafts of a defined shape from autologous chondrocytes. The use of autologous cells minimizes the risk of host immune response; however, factors such as biomaterial compatibility and in vitro maturation of the tissue-engineered (TE) cartilage may influence the engraftment and shape-stability of TE implants. Here we tested the biocompatibility of bioprinted autologous cartilage constructs in a rabbit model. The TE cartilage was produced by embedding autologous auricular chondrocytes into hyaluronan transglutaminase (HATG)-based bioink, previously shown to support chondrogenesis in human auricular chondrocytes in vitro and in immunocompromised xenotransplantation models in vivo. A drastic softening and loss of cartilage markers, such as sulfated glycosaminoglycans and collagen type II were observed. Furthermore, fibrous encapsulation and partial degradation of the transplanted constructs were indicative of a strong host immune response to the autologous TE cartilage. The current study thus illustrates the crucial importance of immunocompetent autologous animal models for the evaluation of TE cartilage function and compatibility. This article is protected by copyright. All rights reserved |
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| Item Description: | Gesehen am 11.03.2024 |
| Physical Description: | Online Resource |
| ISSN: | 2366-3987 |
| DOI: | 10.1002/adtp.202300441 |