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Identification of NAD-RNA species and ADPR-RNA decapping in Archaea

NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archa...

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Main Authors: Gomes-Filho, José Vicente (Author) , Breuer, Ruth (Author) , Morales-Filloy, Hector Gabriel (Author) , Pozhydaieva, Nadiia (Author) , Borst, Andreas (Author) , Paczia, Nicole (Author) , Soppa, Jörg (Author) , Höfer, Katharina (Author) , Jäschke, Andres (Author) , Randau, Lennart (Author)
Format: Article (Journal)
Language:English
Published: 21 November 2023
In: Nature Communications
Year: 2023, Volume: 14, Pages: 1-12
ISSN:2041-1723
DOI:10.1038/s41467-023-43377-x
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1038/s41467-023-43377-x
Verlag, lizenzpflichtig, Volltext: https://www.nature.com/articles/s41467-023-43377-x
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Author Notes:José Vicente Gomes-Filho, Ruth Breuer, Hector Gabriel Morales-Filloy, Nadiia Pozhydaieva, Andreas Borst, Nicole Paczia, Jörg Soppa, Katharina Höfer, Andres Jäschke & Lennart Randau
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Summary:NAD is a coenzyme central to metabolism that also serves as a 5′-terminal cap for bacterial and eukaryotic transcripts. Thermal degradation of NAD can generate nicotinamide and ADP-ribose (ADPR). Here, we use LC-MS/MS and NAD captureSeq to detect and identify NAD-RNAs in the thermophilic model archaeon Sulfolobus acidocaldarius and in the halophilic mesophile Haloferax volcanii. None of the four Nudix proteins of S. acidocaldarius catalyze NAD-RNA decapping in vitro, but one of the proteins (Saci_NudT5) promotes ADPR-RNA decapping. NAD-RNAs are converted into ADPR-RNAs, which we detect in S. acidocaldarius total RNA. Deletion of the gene encoding the 5′−3′ exonuclease Saci-aCPSF2 leads to a 4.5-fold increase in NAD-RNA levels. We propose that the incorporation of NAD into RNA acts as a degradation marker for Saci-aCPSF2. In contrast, ADPR-RNA is processed by Saci_NudT5 into 5′-p-RNAs, providing another layer of regulation for RNA turnover in archaeal cells.
Item Description:Gesehen am 13.03.2024
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/s41467-023-43377-x

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