Ein neues Antikoagulans zur Probengewinnung für Thrombozytenfunktionstests = A new anticoagulant for blood sampling tubes for platelet function tests
Background: The difficult pre-analytical conditions of platelet function diagnostics discourage its widespread use, e.g., to detect resistance to acetylsalicylic acid (ASA) and/or clopidogrel. Platelet activation during blood drawing and sample preparation cannot be prevented by citrate anticoagulat...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | German |
| Published: |
7. Mai 2010
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| In: |
Laboratoriumsmedizin
Year: 2010, Volume: 34, Issue: 3, Pages: 149-153 |
| ISSN: | 1439-0477 |
| DOI: | 10.1515/jlm.2010.026 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1515/jlm.2010.026 Verlag, lizenzpflichtig, Volltext: https://www.degruyterbrill.com/document/doi/10.1515/jlm.2010.026/html 
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| Author Notes: | Johanna Wolf, Martin Sigl, Evangelos Giannitsis und Boris Ivandic |
| Summary: | Background: The difficult pre-analytical conditions of platelet function diagnostics discourage its widespread use, e.g., to detect resistance to acetylsalicylic acid (ASA) and/or clopidogrel. Platelet activation during blood drawing and sample preparation cannot be prevented by citrate anticoagulation. Here, we tested a new blood sampling tube containing the anticoagulant benzylsulfonyl-D-argininyl-prolyl-4-amidinobenzylamide (Thrombovette ® , Probe & go, Osburg, Germany). Methods: A total of 46 emergency patients taking ASA and clopidogrel as well as 20 healthy volunteers were included in the study. Blood was drawn into Thrombovette ® and citrate S-Monovette ® tubes (Sarstedt AG&Co, Nümbrecht, Germany) and transported to the lab via the pneumatic tube system. Whole blood impedance aggregometry (model 560Ca, Chrono-Log, Havertown, PA, USA) was performed using collagen and ADP as platelet agonist (final concentrations 1 mg/L and 5 μM, respectively). Results: Reference ranges and cut-off values for the determination of drug resistance were obtained from the healthy cohort. Positive predictive values were 94.6% and 94.1% for inhibition of aggregation by ASA, and 68.0% and 53.3% for inhibition by clopidogrel using Thrombovette ® and citrate S-Monovette ® , respectively. Negative predictive values were 82.8% and 75.0% for ASA resistance and 87.8% and 83.3% for clopidogrel resistance using Thrombovette ® and citrate S-Monovette ® , respectively. Conclusions: Thrombovette ® is a promising new system for blood sampling and could improve the validity of platelet function diagnostics. |
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| Item Description: | Gesehen am 21.03.2024 |
| Physical Description: | Online Resource |
| ISSN: | 1439-0477 |
| DOI: | 10.1515/jlm.2010.026 |