TNF-α downregulates the peroxisome proliferator activated receptor-α and the mRNAs encoding peroxisomal proteins in rat liver
We have studied the effects of TNF-α on the mRNAs coding for the peroxisome proliferator activated receptor α (PPAR-α), and for catalase (Cat), acyl-CoA oxidase (AOX), multifunctional enzyme (PH), and β-actin in rat liver. Total RNA was isolated from livers of male SD-rats 16 h after administration...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
03 November 1997
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| In: |
FEBS letters
Year: 1997, Volume: 412, Issue: 2, Pages: 385-387 |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/S0014-5793(97)00805-3 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0014-5793(97)00805-3 Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1016/S0014-5793%2897%2900805-3 
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| Author Notes: | Konstantin Beier, Alfred Völkl, H.Dariush Fahimi |
| Summary: | We have studied the effects of TNF-α on the mRNAs coding for the peroxisome proliferator activated receptor α (PPAR-α), and for catalase (Cat), acyl-CoA oxidase (AOX), multifunctional enzyme (PH), and β-actin in rat liver. Total RNA was isolated from livers of male SD-rats 16 h after administration of a single dose of 25 μg TNF-α and mRNAs were analyzed by a novel dot blot RNase protection assay. The mRNAs for PPAR-α and for Cat, AOX and PH were significantly reduced by TNF-treatment. In addition, the level of PPAR-α protein was also decreased after TNF. In contrast, the mRNA for β-actin was markedly increased implying that the effect of TNF on PPAR-α and the peroxisomal mRNAs is highly selective. This effect may have important implications in perturbation of the lipid metabolism induced by TNF-α. |
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| Item Description: | Im Titel ist "alpha" als griechischer Buchstabe dargestellt Gesehen am 25.03.2024 |
| Physical Description: | Online Resource |
| ISSN: | 1873-3468 |
| DOI: | 10.1016/S0014-5793(97)00805-3 |