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Suppression of neutrophils by sodium exacerbates oxidative stress and arthritis

Introduction: Typical Western diet, rich in salt, contributes to autoimmune disease development. However, conflicting reports exist about the effect of salt on neutrophil effector functions, also in the context of arthritis. Methods: We investigated the effect of sodium chloride (NaCl) on neutrophil...

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Main Authors: Zlatar, Leticija (Author) , Mahajan, Aparna (Author) , Muñoz-Becerra, Marco (Author) , Weidner, Daniela (Author) , Bila, Galyna (Author) , Bilyy, Rostyslav (Author) , Titze, Jens (Author) , Hoffmann, Markus H. (Author) , Schett, Georg (Author) , Herrmann, Martin (Author) , Steffen, Ulrike (Author) , Muñoz, Luis E. (Author) , Knopf, Jasmin (Author)
Format: Article (Journal)
Language:English
Published: 02 August 2023
In: Frontiers in immunology
Year: 2023, Volume: 14, Pages: 1-13
ISSN:1664-3224
DOI:10.3389/fimmu.2023.1174537
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3389/fimmu.2023.1174537
Verlag, kostenfrei, Volltext: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1174537/full
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Author Notes:Leticija Zlatar, Aparna Mahajan, Marco Muñoz-Becerra, Daniela Weidner, Galyna Bila, Rostyslav Bilyy, Jens Titze, Markus H. Hoffmann, Georg Schett, Martin Herrmann, Ulrike Steffen, Luis E. Muñoz and Jasmin Knopf
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Summary:Introduction: Typical Western diet, rich in salt, contributes to autoimmune disease development. However, conflicting reports exist about the effect of salt on neutrophil effector functions, also in the context of arthritis. Methods: We investigated the effect of sodium chloride (NaCl) on neutrophil viability and functions in vitro, and in vivo employing the murine K/BxN-serum transfer arthritis (STA) model. Results and discussion: The effects of NaCl and external reactive oxygen species (H2O2) were further examined on osteoclasts in vitro. Hypertonic sodium-rich media caused primary/secondary cell necrosis, altered the nuclear morphology, inhibited phagocytosis, degranulation, myeloperoxidase (MPO) peroxidation activity and neutrophil extracellular trap (NET) formation, while increasing total ROS production, mitochondrial ROS production, and neutrophil elastase (NE) activity. High salt diet (HSD) aggravated arthritis by increasing inflammation, bone erosion, and osteoclast differentiation, accompanied by increased NE expression and activity. Osteoclast differentiation was decreased with 25 mM NaCl or 100 nM H2O2 addition to isotonic media. In contrast to NaCl, external H2O2 had pro-resorptive effects in vitro. We postulate that in arthritis under HSD, increased bone erosion can be attributed to an enhanced oxidative milieu maintained by infiltrating neutrophils, rather than a direct effect of NaCl.
Item Description:Gesehen am 08.04.2024
Physical Description:Online Resource
ISSN:1664-3224
DOI:10.3389/fimmu.2023.1174537

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