Cover Image

An in vitro approach to understand contribution of kidney cells to human urinary extracellular vesicles

Extracellular vesicles (EV) are membranous particles secreted by all cells and found in body fluids. Established EV contents include a variety of RNA species, proteins, lipids and metabolites that are considered to reflect the physiological status of their parental cells. However, to date, little is...

Full description

Saved in:
Bibliographic Details
Main Authors: Barreiro, Karina (Author) , Lay, Abigail C. (Author) , Leparc, German (Author) , Tran, Van Du T. (Author) , Rosler, Marcel (Author) , Dayalan, Lusyan (Author) , Burdet, Frederic (Author) , Ibberson, Mark (Author) , Coward, Richard J. M. (Author) , Huber, Tobias B. (Author) , Krämer, Bernhard (Author) , Delic, Denis (Author) , Holthofer, Harry (Author)
Format: Article (Journal)
Language:English
Published: February 2023
In: Journal of extracellular vesicles
Year: 2023, Volume: 12, Issue: 2, Pages: 1-32
ISSN:2001-3078
DOI:10.1002/jev2.12304
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.1002/jev2.12304
Verlag, kostenfrei, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/jev2.12304
Get full text
Author Notes:Karina Barreiro, Abigail C. Lay, German Leparc, Van Du T. Tran, Marcel Rosler, Lusyan Dayalan, Frederic Burdet, Mark Ibberson, Richard J. M. Coward, Tobias B. Huber, Bernhard K. Krämer, Denis Delic, Harry Holthofer
Description
Summary:Extracellular vesicles (EV) are membranous particles secreted by all cells and found in body fluids. Established EV contents include a variety of RNA species, proteins, lipids and metabolites that are considered to reflect the physiological status of their parental cells. However, to date, little is known about cell-type enriched EV cargo in complex EV mixtures, especially in urine. To test whether EV secretion from distinct human kidney cells in culture differ and can recapitulate findings in normal urine, we comprehensively analysed EV components, (particularly miRNAs, long RNAs and protein) from conditionally immortalised human kidney cell lines (podocyte, glomerular endothelial, mesangial and proximal tubular cells) and compared to EV secreted in human urine. EV from cell culture media derived from immortalised kidney cells were isolated by hydrostatic filtration dialysis (HFD) and characterised by electron microscopy (EM), nanoparticle tracking analysis (NTA) and Western blotting (WB). RNA was isolated from EV and subjected to miRNA and RNA sequencing and proteins were profiled by tandem mass tag proteomics. Representative sets of EV miRNAs, RNAs and proteins were detected in each cell type and compared to human urinary EV isolates (uEV), EV cargo database, kidney biopsy bulk RNA sequencing and proteomics, and single-cell transcriptomics. This revealed that a high proportion of the in vitro EV signatures were also found in in vivo datasets. Thus, highlighting the robustness of our in vitro model and showing that this approach enables the dissection of cell type specific EV cargo in biofluids and the potential identification of cell-type specific EV biomarkers of kidney disease.
Item Description:Online veröffentlicht: 13. Februar 2023
Gesehen am 21.05.2024
Physical Description:Online Resource
ISSN:2001-3078
DOI:10.1002/jev2.12304

Similar Items