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Multimaterial 3D laser printing of cell-adhesive and cell-repellent hydrogels

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Here, a straightforward method is reported for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability, and rapid and precise 3D fabricati...

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Bibliographic Details
Main Authors: Schwegler, Niklas (Author) , Gebert, Tanisha (Author) , Villiou, Maria (Author) , Colombo, Federico (Author) , Schamberger, Barbara (Author) , Selhuber-Unkel, Christine (Author) , Thomas, Franziska (Author) , Blasco, Eva (Author)
Format: Article (Journal)
Language:English
Published: 06 May 2024
In: Small
Year: 2024, Pages: 1-9
ISSN:1613-6829
DOI:10.1002/smll.202401344
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1002/smll.202401344
Verlag, lizenzpflichtig, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202401344
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Author Notes:Niklas Schwegler, Tanisha Gebert, Maria Villiou, Federico Colombo, Barbara Schamberger, Christine Selhuber-Unkel, Franziska Thomas, and Eva Blasco
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Summary:Here, a straightforward method is reported for manufacturing 3D microstructured cell-adhesive and cell-repellent multimaterials using two-photon laser printing. Compared to existing strategies, this approach offers bottom-up molecular control, high customizability, and rapid and precise 3D fabrication. The printable cell-adhesive polyethylene glycol (PEG) based material includes an Arg-Gly-Asp (RGD) containing peptide synthesized through solid-phase peptide synthesis, allowing for precise control of the peptide design. Remarkably, minimal amounts of RGD peptide (< 0.1 wt%) suffice for imparting cell-adhesiveness, while maintaining identical mechanical properties in the 3D printed microstructures to those of the cell-repellent, PEG-based material. Fluorescent labeling of the RGD peptide facilitates visualization of its presence in cell-adhesive areas. To demonstrate the broad applicability of the system, the fabrication of cell-adhesive 2.5D and 3D structures is shown, fostering the adhesion of fibroblast cells within these architectures. Thus, this approach allows for the printing of high-resolution, true 3D structures suitable for diverse applications, including cellular studies in complex environments.
Item Description:Gesehen am 23.05.2024
Physical Description:Online Resource
ISSN:1613-6829
DOI:10.1002/smll.202401344

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