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A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov

Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It...

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Main Authors: Haas, Walter (Author) , Butler, W. R. (Author) , Kirschner, P. (Author) , Plikaytis, B. B. (Author) , Coyle, M. B. (Author) , Amthor, Beate (Author) , Steigerwalt, A. G. (Author) , Brenner, D. J. (Author) , Salfinger, M. (Author) , Crawford, J. T. (Author) , Böttger, E. C. (Author) , Bremer, Hans J. (Author)
Format: Article (Journal)
Language:English
Published: Dec. 1997
In: Journal of clinical microbiology
Year: 1997, Volume: 35, Issue: 12, Pages: 3203-3209
ISSN:1098-660X
DOI:10.1128/jcm.35.12.3203-3209.1997
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1128/jcm.35.12.3203-3209.1997
Verlag, lizenzpflichtig, Volltext: https://journals.asm.org/doi/10.1128/jcm.35.12.3203-3209.1997
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Author Notes:W H Haas, W R Butler, P Kirschner, B B Plikaytis, M B Coyle, B Amthor, A G Steigerwalt, D J Brenner, M Salfinger, J T Crawford, E C Böttger, H J Bremer
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Summary:Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differentiated biochemically from described Mycobacterium species, although it most closely resembled Mycobacterium malmoense by thin-layer chromatography and high-performance liquid chromatography of mycolic acids. A striking characteristic of the isolate was its high degree of susceptibility to antituberculous drugs in vitro, including isoniazid. Direct determination of the 16S rRNA gene sequence revealed a unique sequence and positioned the strain phylogenetically on a branch separate from M. malmoense within a group of slowly growing mycobacteria that show a high degree of similarity to M. simiae at the 16S rRNA gene level. Despite 99.6% sequence identity with M. simiae at the 16S rRNA gene level, DNA-DNA hybridization studies (hydroxyapatite method) demonstrated DNA relatedness of less than 40%. We conclude that this organism is a new species for which we propose the name M. heidelbergense. A culture of the type strain, strain 2554/91, has been deposited in the American Type Culture Collection as strain ATCC 51253.
Item Description:Gesehen am 20.06.2024
Physical Description:Online Resource
ISSN:1098-660X
DOI:10.1128/jcm.35.12.3203-3209.1997

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