Ratiometric fluorescent sensing of pyrophosphate with sp³-functionalized single-walled carbon nanotubes
Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantific...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
24 January 2024
|
| In: |
Nature Communications
Year: 2024, Volume: 15, Pages: 1-13 |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-024-45052-1 |
| Online Access: | Verlag, kostenfrei, Volltext: https://doi.org/10.1038/s41467-024-45052-1 Verlag, kostenfrei, Volltext: https://www.nature.com/articles/s41467-024-45052-1 
|
| Author Notes: | Simon Settele, C. Alexander Schrage, Sebastian Jung, Elena Michel, Han Li, Benjamin S. Flavel, A. Stephen K. Hashmi, Sebastian Kruss & Jana Zaumseil |
| Summary: | Inorganic pyrophosphate is a key molecule in many biological processes from DNA synthesis to cell metabolism. Here we introduce sp3-functionalized (6,5) single-walled carbon nanotubes (SWNTs) with red-shifted defect emission as near-infrared luminescent probes for the optical detection and quantification of inorganic pyrophosphate. The sensing scheme is based on the immobilization of Cu2+ ions on the SWNT surface promoted by coordination to covalently attached aryl alkyne groups and a triazole complex. The presence of Cu2+ ions on the SWNT surface causes fluorescence quenching via photoinduced electron transfer, which is reversed by copper-complexing analytes such as pyrophosphate. The differences in the fluorescence response of sp3-defect to pristine nanotube emission enables reproducible ratiometric measurements in a wide concentration window. Biocompatible, phospholipid-polyethylene glycol-coated SWNTs with such sp3 defects are employed for the detection of pyrophosphate in cell lysate and for monitoring the progress of DNA synthesis in a polymerase chain reaction. This robust ratiometric and near-infrared luminescent probe for pyrophosphate may serve as a starting point for the rational design of nanotube-based biosensors. |
|---|---|
| Item Description: | Gesehen am 21.06.2024 Im Titel ist die Zahl 3 hochgestellt |
| Physical Description: | Online Resource |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-024-45052-1 |