Alterations in cell surface carbohydrate composition of a human colon carcinoma cell line affect adhesion to extracellular matrix components
The adhesion of HT29 human colon adenocarcinoma cells to different extracellular matrix components was studied. While treatment of the cells with sialidase had no detectable effect on binding to laminin and fibronectin, attachment to collagen IV was decreased. However, additional removal of β-(1-4)-...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
January 1992
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| In: |
Biochimie
Year: 1992, Volume: 74, Issue: 1, Pages: 117-122 |
| DOI: | 10.1016/0300-9084(92)90191-G |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/0300-9084(92)90191-G Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/030090849290191G 
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| Author Notes: | W. Kemmner, J. Morgenthaler, R. Brossmer |
| Summary: | The adhesion of HT29 human colon adenocarcinoma cells to different extracellular matrix components was studied. While treatment of the cells with sialidase had no detectable effect on binding to laminin and fibronectin, attachment to collagen IV was decreased. However, additional removal of β-(1-4)-bound galactose led to significantly reduced binding to all of the susbtrates, including fibronectin and laminin. Tunicamycin treatment, monitored by lectin-induced aggregation, drastically diminished cell adhesion to laminin and fibronectin, whereas cell binding to collagen Iv was not affected. Arg-Gly-Asp (RGD)-related peptides were used to study the adhesion to collagen IV. The results show that a serine-containing RGD-related peptide GRGDSP has virtually no effect on colon carcinoma cell adhesion to type IV collagen. In contrast, when serine was substituted for threonine (GRGDTP) adhesion to collagen IV was strongly inhibited. After incubation of sialidase-treated cells with the threonine-containing peptide adhesion was almost totally blocked. These results demonstrate the existence of both RGD-dependent and carbohydrate-based mechanisms for metastatic human HT29 cell binding to collagen IV. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 23. Januar 2003 Gesehen am 24.06.2024 |
| Physical Description: | Online Resource |
| DOI: | 10.1016/0300-9084(92)90191-G |