Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads
Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population c...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
4 March 1992
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| In: |
Journal of immunological methods
Year: 1992, Volume: 147, Issue: 2, Pages: 197-200 |
| ISSN: | 1872-7905 |
| DOI: | 10.1016/S0022-1759(12)80008-1 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1016/S0022-1759(12)80008-1 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0022175912800081 
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| Author Notes: | W. Kemmner, G. Moldenhauer, P. Schlag, R. Brossmer |
| Summary: | Dissociation of colorectal tumor tissue directly obtained at surgery yields a cell suspension which contains tumor cells, leukocytes and erythrocytes. Coupling of a murine monoclonal antibody, HEA125, specific for human epithelial cells, to magnetic beads permits positive selection of a population containing essentially only tumor cells. Removal of cells from the beads is possible by papain treatment. Negative selection of tumor cells by means of beads coated with a leukocyte binding antibody offers no advantage since dead cells and erythrocytes remain. Key words: Cell separation; Colorectal tumor cell; Tumor infiltrating leukocyte; Monoclonal antibody HEA125, epithelium specific; Immunomagnetic bead |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 9. Februar 2012 Gesehen am 25.06.2024 |
| Physical Description: | Online Resource |
| ISSN: | 1872-7905 |
| DOI: | 10.1016/S0022-1759(12)80008-1 |