A synthetic sialic acid analog that is resistant to the receptor-destroying enzyme can be used by influenza C virus as a receptor determinant for infection of cells
A synthetic sialic acid analog, N-acetyl-9-acetamido-9-deoxy-neuraminic acid, can be used by influenza C virus as a receptor determinant for attachment to cells, In contrast to the natural determinant, N-acetyl-9-O-acetylneuraminic acid, the synthetic sialic acid is resistant to the action of the re...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
November 22, 1995
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| In: |
Biochemical and biophysical research communications
Year: 1995, Volume: 216, Issue: 3, Pages: 821-827 |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.1995.2695 |
| Online Access: | Verlag, lizenzpflichtig, Volltext: https://doi.org/10.1006/bbrc.1995.2695 Verlag, lizenzpflichtig, Volltext: https://www.sciencedirect.com/science/article/pii/S0006291X85726952 
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| Author Notes: | Georg Herrler, Hans-Jürgen Gross, and Reinhard Brossmer |
| Summary: | A synthetic sialic acid analog, N-acetyl-9-acetamido-9-deoxy-neuraminic acid, can be used by influenza C virus as a receptor determinant for attachment to cells, In contrast to the natural determinant, N-acetyl-9-O-acetylneuraminic acid, the synthetic sialic acid is resistant to the action of the receptor-destroying acetylesterase of this virus. The sialic acid analog was enzymatically transferred to the surface of Madin-Darby canine kidney cells that are resistant to infection because of a lack of receptors. Influenza C virus was able to infect the modified cells though requiring a 10-fold larger amount of the sialic acid analogue on the cell surface compared to the natural receptor determinant. The quantitative difference is accounted for mainly by a less efficient binding of influenza C virus to the analog. Thus, in our system, inactivation of the receptor by the viral esterase is not required for the initiation of an influenza C virus infection. |
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| Item Description: | Elektronische Reproduktion der Druck-Ausgabe 25. Mai 2002 Gesehen am 27.06.2024 |
| Physical Description: | Online Resource |
| ISSN: | 1090-2104 |
| DOI: | 10.1006/bbrc.1995.2695 |