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Exploring the molecular tapestry: organ-specific peptide and protein ultrafiltrates and their role in therapeutics

This study aims to characterize the proteome composition of organ-derived protein extracts from rabbits. Protein isolation was performed using soft homogenization and size exclusion via ultrafiltration. The proteome analysis of the ultrafiltrates was conducted using gel electrophoresis, and the mass...

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Bibliographic Details
Main Authors: Slivka, Jakub Peter (Author) , Bauer, Chris (Author) , Younsi, Alexander (Author) , Wong, Michelle B. F. (Author) , Chan, Mike K. S. (Author) , Skutella, Thomas (Author)
Format: Article (Journal)
Language:English
Published: 1 March 2024
In: International journal of molecular sciences
Year: 2024, Volume: 25, Issue: 5, Pages: 1-24
ISSN:1422-0067
DOI:10.3390/ijms25052863
Online Access:Verlag, kostenfrei, Volltext: https://doi.org/10.3390/ijms25052863
Verlag, kostenfrei, Volltext: https://www.proquest.com/docview/2955550225/abstract/D28B9A82B17E4C18PQ/1
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Author Notes:Jakub Peter Slivka, Chris Bauer, Alexander Younsi, Michelle B. F. Wong, Mike K. S. Chan and Thomas Skutella
Description
Summary:This study aims to characterize the proteome composition of organ-derived protein extracts from rabbits. Protein isolation was performed using soft homogenization and size exclusion via ultrafiltration. The proteome analysis of the ultrafiltrates was conducted using gel electrophoresis, and the mass spectrometry data were subjected to gene ontology analysis. Proteomic profiling revealed comprehensive protein profiles associated with RNA regulation, fatty acid binding, inflammatory response, oxidative stress, and metabolism. Additionally, our results demonstrate the presence of abundant small proteins, as observed in the mass spectrometry datasets. Small proteins and peptides are crucial in transcription modulation and various biological processes. The protein networks identified in the ultrafiltrates have the potential to enhance and complement biological therapeutic interventions. Data are available via ProteomeXchange with identifier PXD050039.
Item Description:Gesehen am 23.07.2024
Physical Description:Online Resource
ISSN:1422-0067
DOI:10.3390/ijms25052863

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