Cover Image

Chemo- and optogenetic activation of hypothalamic Foxb1-expressing neurons and their terminal endings in the rostral-dorsolateral PAG leads to tachypnea, bradycardia, and immobility

Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray...

Full description

Saved in:
Bibliographic Details
Main Authors: Cola, Reto (Author) , Roccaro-Waldmeyer, Diana M. (Author) , Naim, Samara (Author) , Babalian, Alexandre (Author) , Seebeck, Petra (Author) , Alvarez-Bolado, Gonzalo (Author) , Celio, Marco R. (Author)
Format: Article (Journal)
Language:English
Published: 01 February, 2024
In: eLife
Year: 2024, Volume: 12, Pages: 1-26
ISSN:2050-084X
DOI:10.7554/eLife.86737
Online Access:Verlag, lizenzpflichtig, Volltext: https://doi.org/10.7554/eLife.86737
Get full text
Author Notes:Reto B. Cola, Diana M. Roccaro-Waldmeyer, Samara Naim, Alexandre Babalian, Petra Seebeck, Gonzalo Alvarez-Bolado, Marco R. Celio
Description
Summary:Foxb1 -expressing neurons occur in the dorsal premammillary nucleus (PMd) and further rostrally in the parvafox nucleus, a longitudinal cluster of neurons in the lateral hypothalamus of rodents. The descending projection of these Foxb1+ neurons end in the dorsolateral part of the periaqueductal gray (dlPAG). The functional role of the Foxb1+ neuronal subpopulation in the PMd and the parvafox nucleus remains elusive. In this study, the activity of the Foxb1+ neurons and of their terminal endings in the dlPAG in mice was selectively altered by employing chemo- and optogenetic tools. Our results show that in whole-body barometric plethysmography, hM3Dq-mediated, global Foxb1+ neuron excitation activates respiration. Time-resolved optogenetic gain-of-function manipulation of the terminal endings of Foxb1+ neurons in the rostral third of the dlPAG leads to abrupt immobility and bradycardia. Chemogenetic activation of Foxb1+ cell bodies and ChR2-mediated excitation of their axonal endings in the dlPAG led to a phenotypical presentation congruent with a 'freezing-like' situation during innate defensive behavior.
Item Description:Gesehen am 25.07.2024
Physical Description:Online Resource
ISSN:2050-084X
DOI:10.7554/eLife.86737

Similar Items